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細胞番号 : 細胞名
RCB1464 : DT40  update : 2024/05/01
細胞特性(Comment:英)Chicken B cell line transformed by avian leukosis virus. c-myc and IgM expressing.
細胞特性(日)Avian leukosis virus感染により樹立されたニワトリB細胞腫で、c-myc RNAと細胞表面にIgMを発現する。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Kurosaki, Tomohiro
樹立者 Humphies, H. Eric.
寄託日 1988
動物種 _avian < Birds
採取組織 B cell
病名 avian leukosis virus (ALV)-induced lymphomas
細胞分類 transformed
入手歴 Takeda, Syunnichi ( Kyouto Univ.)
細胞寿命 infinite
細胞形態 lymphocyte-like
Cellosaurus(Expasy) CVCL_0249
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells
培地 RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
培養方法 浮遊細胞の培養に関する一般的な注意
抗生物質 Free
継代方法 dilution
播種細胞数 1.5-2 x 10 5 cells/ml
継代密度 1 : 20 split
継代・培地交換頻度 Subculture : every 2 days
培養最適温度 39.5 ℃
二酸化炭素濃度 5 %
凍結培地 90% FBS + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
ウィルス Avian Influenza Virus (-)
その他 Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 5件
Reference(日) 1件
利用者成果(英) 35件
利用者成果(日) 0件

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Reference(英)
17830  Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H.  Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines  Genes Cells  2015  20(12):1059-76  PubMed ID: 26525166   DOI: 10.1111/gtc.12310
2741  Morrison C, Sonoda E, Takao N, Shinohara A, Yamamoto K, Takeda S  The controlling role of ATM in homologous recombinational repair of DNA damage.  EMBO J  2000  19:463-71  PubMed ID: 10654944   DOI: 10.1093/emboj/19.3.463
2740  Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S  Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells.  EMBO J  1999  18:6619-29  PubMed ID: 10581236   DOI: 10.1093/emboj/18.23.6619
4960  Buerstedde JM, Reynaud CA, Humphries EH, Olson W, Ewert DL, Weill JC.  Light chain gene conversion continues at high rate in an ALV-induced cell line.  EMBO J  1990  9(3):921-7  PubMed ID: 2155784   DOI: 10.1002/j.1460-2075.1990.tb08190.x
2285  Baba, T W, Giroir, B P, Humphries, E H  Cell lines derived from avian lymphomas exhibit two distinct phenotypes.  Virology  1985  144:139-51  PubMed ID: 2998040   DOI: 10.1016/0042-6822(85)90312-5

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Reference(日)
2418  石合正道、黒崎知博  新たなアダプター分子BLNK  免疫1999-2000  1999  30-36   

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利用者成果(英)
19601  Singaram I, Sharma A, Pant S, Lihan M, Park MJ, Pergande M, Buwaneka P, Hu Y, Mahmud N, Kim YM, Cologna S, Gevorgyan V, Khan I, Tajkhorshid E, Cho W.  Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia  Nat Chem Biol  2023  19(2):239-250  PubMed ID: 36229686   DOI: 10.1038/s41589-022-01150-z
16621  Dhami K, Chakraborty A, Gururaja TL, Cheung LW, Sun C, DeAnda F, Huang X.  Kinase-deficient BTK mutants confer ibrutinib resistance through activation of the kinase HCK  Sci Signal   2022  15(736):eabg5216  PubMed ID: 35639855   DOI: 10.1126/scisignal.abg5216
16819  Horia Vais, Min Wang, Karthik Mallilankaraman, Riley Payne, Chris McKennan, Jeffrey T Lock, Lynn A Spruce, Carly Fiest, Matthew Yan-lok Chan, Ian Parker, Steven H Seeholzer, J Kevin Foskett, Don-On Daniel Mak  ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein  eLife  2020  9  PubMed ID: 32420875   DOI: 10.7554/elife.53531
11957  Rodríguez-Fdez S, Citterio C, Lorenzo-Martín LF, Baltanás-Copado J, Llorente-González C, Corbalán-García S, Vicente-Manzanares M, Bustelo XR.  Phosphatidylinositol Monophosphates Regulate Optimal Vav1 Signaling Output.  Cells  2019    PubMed ID: 31888228   DOI: 10.3390/cells8121649
10136  Kawaguchi Y, Nariki H, Kawamoto N, Kanehiro Y, Miyazaki S, Suzuki M, Magari M, Tokumitsu H, Kanayama N.  SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus.  Biochem. Biophys. Res. Commun.  2017  485:261-266  PubMed ID: 28235482   DOI: 10.1016/j.bbrc.2017.02.097
10282  Katow H, Kanaya T, Ogawa T, Egawa R, Yawo H.  Regulation of axon arborization pattern in the developing chick ciliary ganglion: Possible involvement of caspase 3.  Dev. Growth Differ.  2017  59:115-128  PubMed ID: 28430358   DOI: 10.1111/dgd.12346
7115  Zaldua N, Llavero F, Artaso A, Galvez P, Lacerda HM, Parada LA, Zugaza JL.  Rac1/p21-activated kinase pathway controls retinoblastoma protein phosphorylation and E2F transcription factor activation in B lymphocytes.  FEBS J.  2016  283:647-61  PubMed ID: 26663827   DOI: 10.1111/febs.13617
14716  Fujita T, Yuno M, Fujii H.  Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins  Genes Cells  2016  21(4):370-7  PubMed ID: 26848818   DOI: 10.1111/gtc.12341
15039  Walliser C, Tron K, Clauss K, Gutman O, Kobitski AY, Retlich M, Schade A, Röcker C, Henis YI, Nienhaus GU, Gierschik P.  Rac-mediated Stimulation of Phospholipase Cγ2 Amplifies B Cell Receptor-induced Calcium Signaling  J Biol Chem  2015  290(28):17056-72  PubMed ID: 25903139   DOI: 10.1074/jbc.M115.645739
15165  MacVicar TD, Mannack LV, Lees RM, Lane JD.  Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells  Int J Mol Sci   2015  16(6):13356-80  PubMed ID: 26110381   DOI: 10.3390/ijms160613356
15197  Liu TM, Woyach JA, Zhong Y, Lozanski A, Lozanski G, Dong S, Strattan E, Lehman A, Zhang X, Jones JA, Flynn J, Andritsos LA, Maddocks K, Jaglowski SM, Blum KA, Byrd JC, Dubovsky JA, Johnson AJ.  Hypermorphic mutation of phospholipase C, γ2 acquired in ibrutinib-resistant CLL confers BTK independency upon B-cell receptor activation  Blood  2015  126(1):61-8  PubMed ID: 25972157   DOI: 10.1182/blood-2015-02-626846
15204  atterson HC, Gerbeth C, Thiru P, Vögtle NF, Knoll M, Shahsafaei A, Samocha KE, Huang CX, Harden MM, Song R, Chen C, Kao J, Shi J, Salmon W, Shaul YD, Stokes MP, Silva JC, Bell GW, MacArthur DG, Ruland J, Meisinger C, Lodish HF.  A respiratory chain controlled signal transduction cascade in the mitochondrial intermembrane space mediates hydrogen peroxide signaling  Proc Natl Acad Sci U S A   2015  112(42):E5679-8  PubMed ID: 26438848   DOI: 10.1073/pnas.1517932112
17120  Fujita T, Fujii H.  Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins  BMC Mol Biol  2014  15:26  PubMed ID: 25428274   DOI: 10.1186/s12867-014-0026-0
16932  Manami Watanabe, Yuta Kamada, Khosuke Miyazaki, Shoko Mizoguchi, Keiichi Matsuzaki, Susumu Kitanaka, Shohei Miyata  20-O-IngenolEZ, a catalytic topoisomerase II inhibitor, specifically inhibits cell proliferation and induces double-strand DNA breaks in BLM-/- cells  Med Chem Comm  2011  2:824    DOI: 10.1039/c0md00252f
8442  Liu Z, Mai A, Sun J.  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  J. Immunol.  2010  184:244-54  PubMed ID: 19949111   DOI: 10.4049/jimmunol.0902324
19068  Kajita M, Okazawa T, Ikeda M, Todo K, Magari M, Kanayama N, Ohmori H.  Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation  J Biosci Bioeng  2010  110(3):351-8  PubMed ID: 20547352   DOI: 10.1016/j.jbiosc.2010.03.006
15634  Rahman T, Taylor CW.  Nuclear patch-clamp recording from inositol 1,4,5-trisphosphate receptors  Methods Cell Biol  2010  99:199-224  PubMed ID: 21035688   DOI: 10.1016/B978-0-12-374841-6.00008-6
8598  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601   DOI: 10.1371/journal.pgen.1000461
18450  Kochi Y, Myouzen K, Yamada R, Suzuki A, Kurosaki T, Nakamura Y, Yamamoto K.  FCRL3, an autoimmune susceptibility gene, has inhibitory potential on B-cell receptor-mediated signaling  J Immunol  2009  183(9):5502-10  PubMed ID: 19843936   DOI: 10.4049/jimmunol.0901982
18425  Caloca MJ, Zugaza JL, Bustelo XR.  Mechanistic analysis of the amplification and diversification events induced by Vav proteins in B-lymphocytes  J Biol Chem . 2008 Dec 26;283(52):36454-64  2008  J Biol Chem . 2008 Dec 26;283(52):36454-64  PubMed ID: 18974050   DOI: 10.1074/jbc.M803814200
9070  Kaori Terasaki, Haruko Hirayama, Christopher J Kasanga, Min Thein Maw, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi  Chicken B Lymphoma DT40 Cells as a Useful Tool for in Vitro Analysis of Pathogenic Infectious Bursal Disease Virus  J Vet Med Sci  2008  70 (4), 407-10  PubMed ID: 18460838   DOI: 10.1292/jvms.70.407
8809  Honda H, Ichiyanagi K, Suzuki J, Ono T, Koyama H, Kajikawa M, Okada N.  A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics.  Gene  2007  395:116-24  PubMed ID: 17434692   DOI: 10.1016/j.gene.2007.02.017
2876  Kanayama N, Todo K, Reth M, Ohmori H  Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line.  Biochem Biophys Res Commun  2005  327:70-5  PubMed ID: 15629431   DOI: 10.1016/j.bbrc.2004.11.143
3371  Lievremont, Jean-Philippe, Numaga, Takuro, Vazquez, Guillermo, Lemonnier, Loic, Hara, Yuji, Mori, Emiko, Trebak, Mohamed, Moss, Stephen E, Bird, Gary S, Mori, Yasuo, Putney, James W Jr  The role of canonical transient receptor potential 7 in B-cell receptor-activated channels.  J Biol Chem  2005  280:35346-51  PubMed ID: 16123040   DOI: 10.1074/jbc.M507606200
3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039   DOI: 10.1667/rr3239
9254  Iwashita H, Yoshida M, Nishi T, Otani M, Ueda S.  In vivo transfer of a neuronal nitric oxide synthase expression vector into the rat bladder by electroporation.  BJU Int.  2004  93:1098-103  PubMed ID: 15142171   DOI: 10.1111/j.1464-410X.2003.04788.x
18641  Qin S, Chock PB.  Implication of phosphatidylinositol 3-kinase membrane recruitment in hydrogen peroxide-induced activation of PI3K and Akt  Biochemistry  2003  42(10):2995-3003  PubMed ID: 12627965   DOI: 10.1021/bi0205911
9376  Wedel BJ, Vazquez G, McKay RR, St J Bird G, Putney JW Jr.  A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process.  J. Biol. Chem.  2003  278:25758-65  PubMed ID: 12730194   DOI: 10.1074/jbc.M303890200
9379  Vazquez G, Wedel BJ, Trebak M, St John Bird G, Putney JW Jr.  Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation.  J. Biol. Chem.  2003  278:21649-54  PubMed ID: 12686562   DOI: 10.1074/jbc.M302162200
4398  Catley L, Weisberg E, Tai YT, Atadja P, Remiszewski S, Hideshima T, Mitsiades N, Shringarpure R, LeBlanc R, Chauhan D, Munshi NC, Schlossman R, Richardson P, Griffin J, Anderson KC.  NVP-LAQ824 is a potent novel histone deacetylase inhibitor with significant activity against multiple myeloma.  Blood  2003  102(7):2615-22  PubMed ID: 12816865   DOI: 10.1182/blood-2003-01-0233
9416  Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW.  An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes.  EMBO J.  2002  21:4531-8  PubMed ID: 12198155   DOI: 10.1093/emboj/cdf467
9434  Trebak M, Bird GS, McKay RR, Putney JW Jr.  Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Differential sensitivity to channel blockers suggests fundamental differences in channel composition.  J. Biol. Chem.  2002  277:21617-23  PubMed ID: 11943785   DOI: 10.1074/jbc.M202549200
9450  Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr.  Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.  Proc. Natl. Acad. Sci. U.S.A.  2001  98:11777-82  PubMed ID: 11553786   DOI: 10.1073/pnas.201238198
9452  Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr.  Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.  J. Biol. Chem.  2001  276:15945-52  PubMed ID: 11278938   DOI: 10.1074/jbc.M011571200
16950  Newman DK, Hamilton C, Newman PJ.  Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck)  Blood  2001  97(8):2351-7  PubMed ID: 11290597   DOI: 10.1182/blood.v97.8.2351

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