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Cell No. : Cell Name
RCB1464 : DT40  update : 2023/06/30
CommentChicken B cell line transformed by avian leukosis virus. c-myc and IgM expressing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kurosaki, Tomohiro
Originator Humphies, H. Eric.
Year of deposit 1988
Animal _avian < Birds
Tissue B cell
Disease name avian leukosis virus (ALV)-induced lymphomas
Classification transformed
History Takeda, Syunnichi ( Kyouto Univ.)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0249
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus Avian Influenza Virus (-)
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
deposit info
lot info
Reference information Reference 5
User's Publication 30

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17830  Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H.  Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines  Genes Cells  2015  20(12):1059-76  PubMed ID: 26525166   DOI: 10.1111/gtc.12310
2741  Morrison C, Sonoda E, Takao N, Shinohara A, Yamamoto K, Takeda S  The controlling role of ATM in homologous recombinational repair of DNA damage.  EMBO J  2000  19:463-71  PubMed ID: 10654944   DOI: 10.1093/emboj/19.3.463
2740  Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S  Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells.  EMBO J  1999  18:6619-29  PubMed ID: 10581236   DOI: 10.1093/emboj/18.23.6619
4960  Buerstedde JM, Reynaud CA, Humphries EH, Olson W, Ewert DL, Weill JC.  Light chain gene conversion continues at high rate in an ALV-induced cell line.  EMBO J  1990  9(3):921-7  PubMed ID: 2155784   DOI: 10.1002/j.1460-2075.1990.tb08190.x
2285  Baba, T W, Giroir, B P, Humphries, E H  Cell lines derived from avian lymphomas exhibit two distinct phenotypes.  Virology  1985  144:139-51  PubMed ID: 2998040   DOI: 10.1016/0042-6822(85)90312-5

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User's Publication
16621  Dhami K, Chakraborty A, Gururaja TL, Cheung LW, Sun C, DeAnda F, Huang X.  Kinase-deficient BTK mutants confer ibrutinib resistance through activation of the kinase HCK  Sci Signal   2022  15(736):eabg5216  PubMed ID: 35639855   DOI: 10.1126/scisignal.abg5216
16819  Horia Vais, Min Wang, Karthik Mallilankaraman, Riley Payne, Chris McKennan, Jeffrey T Lock, Lynn A Spruce, Carly Fiest, Matthew Yan-lok Chan, Ian Parker, Steven H Seeholzer, J Kevin Foskett, Don-On Daniel Mak  ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein  eLife  2020  9  PubMed ID: 32420875   DOI: 10.7554/elife.53531
11957  Rodríguez-Fdez S, Citterio C, Lorenzo-Martín LF, Baltanás-Copado J, Llorente-González C, Corbalán-García S, Vicente-Manzanares M, Bustelo XR.  Phosphatidylinositol Monophosphates Regulate Optimal Vav1 Signaling Output.  Cells  2019    PubMed ID: 31888228   DOI: 10.3390/cells8121649
10136  Kawaguchi Y, Nariki H, Kawamoto N, Kanehiro Y, Miyazaki S, Suzuki M, Magari M, Tokumitsu H, Kanayama N.  SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus.  Biochem. Biophys. Res. Commun.  2017  485:261-266  PubMed ID: 28235482   DOI: 10.1016/j.bbrc.2017.02.097
10282  Katow H, Kanaya T, Ogawa T, Egawa R, Yawo H.  Regulation of axon arborization pattern in the developing chick ciliary ganglion: Possible involvement of caspase 3.  Dev. Growth Differ.  2017  59:115-128  PubMed ID: 28430358   DOI: 10.1111/dgd.12346
7115  Zaldua N, Llavero F, Artaso A, Galvez P, Lacerda HM, Parada LA, Zugaza JL.  Rac1/p21-activated kinase pathway controls retinoblastoma protein phosphorylation and E2F transcription factor activation in B lymphocytes.  FEBS J.  2016  283:647-61  PubMed ID: 26663827   DOI: 10.1111/febs.13617
14716  Fujita T, Yuno M, Fujii H.  Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins  Genes Cells  2016  21(4):370-7  PubMed ID: 26848818   DOI: 10.1111/gtc.12341
15039  Walliser C, Tron K, Clauss K, Gutman O, Kobitski AY, Retlich M, Schade A, Röcker C, Henis YI, Nienhaus GU, Gierschik P.  Rac-mediated Stimulation of Phospholipase Cγ2 Amplifies B Cell Receptor-induced Calcium Signaling  J Biol Chem  2015  290(28):17056-72  PubMed ID: 25903139   DOI: 10.1074/jbc.M115.645739
15165  MacVicar TD, Mannack LV, Lees RM, Lane JD.  Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells  Int J Mol Sci   2015  16(6):13356-80  PubMed ID: 26110381   DOI: 10.3390/ijms160613356
15197  Liu TM, Woyach JA, Zhong Y, Lozanski A, Lozanski G, Dong S, Strattan E, Lehman A, Zhang X, Jones JA, Flynn J, Andritsos LA, Maddocks K, Jaglowski SM, Blum KA, Byrd JC, Dubovsky JA, Johnson AJ.  Hypermorphic mutation of phospholipase C, γ2 acquired in ibrutinib-resistant CLL confers BTK independency upon B-cell receptor activation  Blood  2015  126(1):61-8  PubMed ID: 25972157   DOI: 10.1182/blood-2015-02-626846
15204  atterson HC, Gerbeth C, Thiru P, Vögtle NF, Knoll M, Shahsafaei A, Samocha KE, Huang CX, Harden MM, Song R, Chen C, Kao J, Shi J, Salmon W, Shaul YD, Stokes MP, Silva JC, Bell GW, MacArthur DG, Ruland J, Meisinger C, Lodish HF.  A respiratory chain controlled signal transduction cascade in the mitochondrial intermembrane space mediates hydrogen peroxide signaling  Proc Natl Acad Sci U S A   2015  112(42):E5679-8  PubMed ID: 26438848   DOI: 10.1073/pnas.1517932112
17120  Fujita T, Fujii H.  Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins  BMC Mol Biol  2014  15:26  PubMed ID: 25428274   DOI: 10.1186/s12867-014-0026-0
16932  Manami Watanabe, Yuta Kamada, Khosuke Miyazaki, Shoko Mizoguchi, Keiichi Matsuzaki, Susumu Kitanaka, Shohei Miyata  20-O-IngenolEZ, a catalytic topoisomerase II inhibitor, specifically inhibits cell proliferation and induces double-strand DNA breaks in BLM-/- cells  Med Chem Comm  2011  2:824    DOI: 10.1039/c0md00252f
8442  Liu Z, Mai A, Sun J.  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  J. Immunol.  2010  184:244-54  PubMed ID: 19949111   DOI: 10.4049/jimmunol.0902324
15634  Rahman T, Taylor CW.  Nuclear patch-clamp recording from inositol 1,4,5-trisphosphate receptors  Methods Cell Biol  2010  99:199-224  PubMed ID: 21035688   DOI: 10.1016/B978-0-12-374841-6.00008-6
8598  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601   DOI: 10.1371/journal.pgen.1000461
9070  Kaori Terasaki, Haruko Hirayama, Christopher J Kasanga, Min Thein Maw, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi  Chicken B Lymphoma DT40 Cells as a Useful Tool for in Vitro Analysis of Pathogenic Infectious Bursal Disease Virus  J Vet Med Sci  2008  70 (4), 407-10  PubMed ID: 18460838   DOI: 10.1292/jvms.70.407
8809  Honda H, Ichiyanagi K, Suzuki J, Ono T, Koyama H, Kajikawa M, Okada N.  A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics.  Gene  2007  395:116-24  PubMed ID: 17434692   DOI: 10.1016/j.gene.2007.02.017
2876  Kanayama N, Todo K, Reth M, Ohmori H  Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line.  Biochem Biophys Res Commun  2005  327:70-5  PubMed ID: 15629431   DOI: 10.1016/j.bbrc.2004.11.143
3371  Lievremont, Jean-Philippe, Numaga, Takuro, Vazquez, Guillermo, Lemonnier, Loic, Hara, Yuji, Mori, Emiko, Trebak, Mohamed, Moss, Stephen E, Bird, Gary S, Mori, Yasuo, Putney, James W Jr  The role of canonical transient receptor potential 7 in B-cell receptor-activated channels.  J Biol Chem  2005  280:35346-51  PubMed ID: 16123040   DOI: 10.1074/jbc.M507606200
3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039   DOI: 10.1667/rr3239
9254  Iwashita H, Yoshida M, Nishi T, Otani M, Ueda S.  In vivo transfer of a neuronal nitric oxide synthase expression vector into the rat bladder by electroporation.  BJU Int.  2004  93:1098-103  PubMed ID: 15142171   DOI: 10.1111/j.1464-410X.2003.04788.x
9376  Wedel BJ, Vazquez G, McKay RR, St J Bird G, Putney JW Jr.  A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process.  J. Biol. Chem.  2003  278:25758-65  PubMed ID: 12730194   DOI: 10.1074/jbc.M303890200
9379  Vazquez G, Wedel BJ, Trebak M, St John Bird G, Putney JW Jr.  Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation.  J. Biol. Chem.  2003  278:21649-54  PubMed ID: 12686562   DOI: 10.1074/jbc.M302162200
4398  Catley L, Weisberg E, Tai YT, Atadja P, Remiszewski S, Hideshima T, Mitsiades N, Shringarpure R, LeBlanc R, Chauhan D, Munshi NC, Schlossman R, Richardson P, Griffin J, Anderson KC.  NVP-LAQ824 is a potent novel histone deacetylase inhibitor with significant activity against multiple myeloma.  Blood  2003  102(7):2615-22  PubMed ID: 12816865   DOI: 10.1182/blood-2003-01-0233
9416  Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW.  An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes.  EMBO J.  2002  21:4531-8  PubMed ID: 12198155   DOI: 10.1093/emboj/cdf467
9434  Trebak M, Bird GS, McKay RR, Putney JW Jr.  Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Differential sensitivity to channel blockers suggests fundamental differences in channel composition.  J. Biol. Chem.  2002  277:21617-23  PubMed ID: 11943785   DOI: 10.1074/jbc.M202549200
9450  Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr.  Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.  Proc. Natl. Acad. Sci. U.S.A.  2001  98:11777-82  PubMed ID: 11553786   DOI: 10.1073/pnas.201238198
9452  Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr.  Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.  J. Biol. Chem.  2001  276:15945-52  PubMed ID: 11278938   DOI: 10.1074/jbc.M011571200
16950  Newman DK, Hamilton C, Newman PJ.  Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck)  Blood  2001  97(8):2351-7  PubMed ID: 11290597   DOI: 10.1182/blood.v97.8.2351

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