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Cell No. : Cell Name
RCB1464 : DT40  update : 2020/09/24
CommentChicken B cell line transformed by avian leukosis virus. c-myc and IgM expressing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kurosaki, Tomohiro
Originator Humphies, H. Eric.
Year of deposit 1988
Animal avian < Birds
Tissue B cell
Case history avian leukosis virus (ALV)-induced lymphomas
Classification transformed
History Takeda, Syunnichi ( Kyouto Univ.)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus Avian Influenza Virus (-)
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
deposit info
lot info
Reference information Reference 4
User's Publication 19

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2741  Morrison C, Sonoda E, Takao N, Shinohara A, Yamamoto K, Takeda S  The controlling role of ATM in homologous recombinational repair of DNA damage.  EMBO J  2000  19:463-71  PubMed ID: 10654944
2740  Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S  Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells.  EMBO J  1999  18:6619-29  PubMed ID: 10581236
4960  Buerstedde JM, Reynaud CA, Humphries EH, Olson W, Ewert DL, Weill JC.  Light chain gene conversion continues at high rate in an ALV-induced cell line.  EMBO J  1990  9(3):921-7  PubMed ID: 2155784
2285  Baba, T W, Giroir, B P, Humphries, E H  Cell lines derived from avian lymphomas exhibit two distinct phenotypes.  Virology  1985  144:139-51  PubMed ID: 2998040

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User's Publication
11957  Rodríguez-Fdez S, Citterio C, Lorenzo-Martín LF, Baltanás-Copado J, Llorente-González C, Corbalán-García S, Vicente-Manzanares M, Bustelo XR.  Phosphatidylinositol Monophosphates Regulate Optimal Vav1 Signaling Output.  Cells  2019    PubMed ID: 31888228
10136  Kawaguchi Y, Nariki H, Kawamoto N, Kanehiro Y, Miyazaki S, Suzuki M, Magari M, Tokumitsu H, Kanayama N.  SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus.  Biochem. Biophys. Res. Commun.  2017  485:261-266  PubMed ID: 28235482
10282  Katow H, Kanaya T, Ogawa T, Egawa R, Yawo H.  Regulation of axon arborization pattern in the developing chick ciliary ganglion: Possible involvement of caspase 3.  Dev. Growth Differ.  2017  59:115-128  PubMed ID: 28430358
7115  Zaldua N, Llavero F, Artaso A, Galvez P, Lacerda HM, Parada LA, Zugaza JL.  Rac1/p21-activated kinase pathway controls retinoblastoma protein phosphorylation and E2F transcription factor activation in B lymphocytes.  FEBS J.  2015  283:647-61  PubMed ID: 26663827
8442  Liu Z, Mai A, Sun J.  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  J. Immunol.  2009  184:244-54  PubMed ID: 19949111
8598  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601
9070  Kaori Terasaki, Haruko Hirayama, Christopher J Kasanga, Min Thein Maw, Kenji Ohya, Tsuyoshi Yamaguchi, Hideto Fukushi  Chicken B Lymphoma DT40 Cells as a Useful Tool for in Vitro Analysis of Pathogenic Infectious Bursal Disease Virus  J Vet Med Sci  2008  70 (4), 407-10  PubMed ID: 18460838
8809  Honda H, Ichiyanagi K, Suzuki J, Ono T, Koyama H, Kajikawa M, Okada N.  A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics.  Gene  2007  395:116-24  PubMed ID: 17434692
2876  Kanayama N, Todo K, Reth M, Ohmori H  Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line.  Biochem Biophys Res Commun  2005  327:70-5  PubMed ID: 15629431
3371  Lievremont, Jean-Philippe, Numaga, Takuro, Vazquez, Guillermo, Lemonnier, Loic, Hara, Yuji, Mori, Emiko, Trebak, Mohamed, Moss, Stephen E, Bird, Gary S, Mori, Yasuo, Putney, James W Jr  The role of canonical transient receptor potential 7 in B-cell receptor-activated channels.  J Biol Chem  2005  280:35346-51  PubMed ID: 16123040
3086  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Radiat Res  2004  162:433-41  PubMed ID: 15447039
9254  Iwashita H, Yoshida M, Nishi T, Otani M, Ueda S.  In vivo transfer of a neuronal nitric oxide synthase expression vector into the rat bladder by electroporation.  BJU Int.  2004  93:1098-103  PubMed ID: 15142171
9376  Wedel BJ, Vazquez G, McKay RR, St J Bird G, Putney JW Jr.  A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process.  J. Biol. Chem.  2003  278:25758-65  PubMed ID: 12730194
9379  Vazquez G, Wedel BJ, Trebak M, St John Bird G, Putney JW Jr.  Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation.  J. Biol. Chem.  2003  278:21649-54  PubMed ID: 12686562
4398  Catley L, Weisberg E, Tai YT, Atadja P, Remiszewski S, Hideshima T, Mitsiades N, Shringarpure R, LeBlanc R, Chauhan D, Munshi NC, Schlossman R, Richardson P, Griffin J, Anderson KC.  NVP-LAQ824 is a potent novel histone deacetylase inhibitor with significant activity against multiple myeloma.  Blood  2003  102(7):2615-22  PubMed ID: 12816865
9416  Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW.  An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes.  EMBO J.  2002  21:4531-8  PubMed ID: 12198155
9434  Trebak M, Bird GS, McKay RR, Putney JW Jr.  Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Differential sensitivity to channel blockers suggests fundamental differences in channel composition.  J. Biol. Chem.  2002  277:21617-23  PubMed ID: 11943785
9450  Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr.  Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.  Proc. Natl. Acad. Sci. U.S.A.  2001  98:11777-82  PubMed ID: 11553786
9452  Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr.  Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.  J. Biol. Chem.  2001  276:15945-52  PubMed ID: 11278938

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