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Cell No. : Cell Name
RCB1460 : PCM6  update : 2021/05/21
CommentHuman myeloma cell.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Minami(Hirose), Rumi
Originator Takahira, Hiroyuki
Year of deposit 1998
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 60 years
Tissue peripheral blood
Disease name myeloma
Classification cancer
Year of origin 1992
History originator -> Minami(Hirose), R.
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4666
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives McCoy's 5A + 20% FBS + 30U/ml human IL-6
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 44(50)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
2677  Takahira H, Kozuru M, Hirata J, Obama K, Uike N, Iguchi H, Miyamura T, Yamashita S, Kono A, Umemura T  Establishment of a human myeloma cell line with growth-promoting activity for bone marrow-derived fibroblastoid colony-forming cells.  Exp Hematol  1994  22:261-6  PubMed ID: 8112425

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User's Publication
6968  Suzuki T, Sato Y, Sano K, Arashiro T, Katano H, Nakajima N, Shimojima M, Kataoka M, Takahashi K, Wada Y, Morikawa S, Fukushi S, Yoshikawa T, Saijo M, Hasegawa H.  Severe fever with thrombocytopenia syndrome virus targets B cells in lethal human infections.  J Clin Invest  2020  130(2):799-812  PubMed ID: 31904586
7595  Harada Y, Ishii I, Hatake K, Kasahara T.  Pyrvinium pamoate inhibits proliferation of myeloma/erythroleukemia cells by suppressing mitochondrial respiratory complex I and STAT3.  Cancer Lett.  2012  319:83-8  PubMed ID: 22210382



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