Cell No. : Cell Name
RCB1456 : RCR-1
update : 2022/06/30
|
| Comment | Astroglia cell line. Possible to culture in DMEM medium. See RCB0075. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Jou,Teh-Cheng et al.
|
| Year of deposit |
1998
|
| Original cell |
RCB0075
|
| Animal |
_rat
< Mammals
|
| Age at sampling |
3 days
|
| Tissue |
brain
|
| Classification |
other
|
| Memo_1 |
RCB0075 : Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_4856
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
2
|
| User's Publication |
|
21036
Sato K, Suzuki-Utsunomiya K, Mitsui S, Ono S, Shimakura K, Otomo A, Hadano S.
Central nervous system specific high molecular weight ALS2/alsin homophilic complex is enriched in mouse brain synaptosomes.
Biochem Biophys Res Commun
2023
638:168-175
PubMed ID: 36459881
DOI: 10.1016/j.bbrc.2022.11.061
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4959
Oishi Y, Uezono Y, Yanagihara N, Izumi F, Nakamura T, Suzuki K.
Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytoma RCR-1 cells.
Brain Res
1998
781(1-2):159-66
PubMed ID: 9507103
DOI: 10.1016/s0006-8993(97)01226-2
|
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