Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1456 : RCR-1  update : 2022/06/30
CommentAstroglia cell line. Possible to culture in DMEM medium. See RCB0075.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Jou,Teh-Cheng et al.
Year of deposit 1998
Original cell RCB0075
Animal rat < Mammals
Age at sampling 3 days
Tissue brain
Classification other
Memo_1 RCB0075 : Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4856
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


To topTop
Reference
994  Jou TC, Takahashi M, Yasuda SI, Takaoka T.  Immunofluorescence characterization of astrocytes in monolayer culture using antiserum to glial fibrillary acidic protein.  Taiwan Yi Xue Hui Za Zhi  1983  82(11):1115-25  PubMed ID: 6368741  

To topTop
User's Publication
4959  Oishi Y, Uezono Y, Yanagihara N, Izumi F, Nakamura T, Suzuki K.  Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytoma RCR-1 cells.  Brain Res  1998  781(1-2):159-66  PubMed ID: 9507103   DOI: 10.1016/s0006-8993(97)01226-2
4958  Kobayashi H, Mizuki T, Tsutsui M, Minami K, Yanagihara N, Yuhi T, Izumi F.  Receptors for C-type natriuretic peptide in cultured rat glial cells.  Brain Res  1993  617(1):163-6  PubMed ID: 8104082   DOI: 10.1016/0006-8993(93)90630-6



Back Back Return Top Page