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Cell No. : Cell Name
RCB1451 : L929  update : 2022/04/04
CommentSanford's original, brought by Katsuta at 7/5/1955. Insensitive to TNFalpha. Possible to culture in MEM medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Year of deposit 1998
Animal mouse < Mammals
Genus Mus
Species musculus
Tissue connective tissue
Classification other
History RCB0081
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0462
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 5% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 33

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537  Stanley ER, Heard PM.  Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.  J Biol Chem  1977  252(12):4305-12  PubMed ID: 301140
4661  WAYMOUTH C.  Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).  J Natl Cancer Inst  1959  22(5):1003-17  PubMed ID: 13655076
1817  SANFORD KK, HOBBS GL, EARLE WR.  The tumor-producing capacity of strain L mouse cells after 10 years in vitro.  Cancer Res  1956  16(2):162-6  PubMed ID: 13293658
4660  EAGLE H.  The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.  J Exp Med  1955  102(5):595-600  PubMed ID: 13271674
1818  EARLE WR, SCHILLING EL, BRYANT JC, EVANS VJ.  The growth of pure strain L cells in fluid-suspension cultures.  J Natl Cancer Inst.  1954  14(5):1159-71  PubMed ID: 13233852
4659  EVANS VJ, SHANNON JE Jr, BRYANT JC, WALTZ HK, EARLE WR, SANFORD KK.  A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.  J Natl Cancer Inst  1953  13(4):773-84  PubMed ID: 13035407
4658  SANFORD KK, EARLE WR, LIKELY GD.  The growth in vitro of single isolated tissue cells.  J Natl Cancer Inst  1948  9(3):229-46  PubMed ID: 18105872
4657  Earle, W.R  Production of malignancy in vitro.Ⅳ.The mouse fibroblast cultures and changes seen in the living cells.  J. Nat. Cancer Inst.  1943  4:165-212 

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User's Publication
20519  Evans C, Morimitsu Y, Nishi R, Yoshida M, Takei T.  Novel hydrophobically modified agarose cryogels fabricated using dimethyl sulfoxide.  J Biosci Bioeng  2022    PubMed ID: 35031212
20111  Evans C, Morimitsu Y, Hisadome T, Inomoto F, Yoshida M, Takei T.  Optimized hydrophobically modified chitosan cryogels for strength and drug delivery systems.  J Biosci Bioeng  2021    PubMed ID: 33853755
20190  Ishihara K, Fukazawa K, Sharma V, Liang S, Shows A, Dunbar DC, Zheng Y, Ge J, Zhang S, Hong Y, Shi X, Wu JY.  Antifouling Silicone Hydrogel Contact Lenses with a Bioinspired 2-Methacryloyloxyethyl Phosphorylcholine Polymer Surface.  ACS Omega  2021  6(10):7058-7067  PubMed ID: 33748619
14513  Yudai Futaki, Ikumi Amimoto, Megumi Tanaka, Tomoki Ito, Yoshiaki Hirano  Discovery of Cell Aggregate-Inducing Peptides  Discovery of Cell Aggregate-Inducing Peptides  2021  9:538 
15681  Daisuke Fujii, Kento Takase, Ami Takagi, Kei Kamino, Yoshiaki Hirano  Design of RGDS Peptide-Immobilized Self-Assembling β-Strand Peptide from Barnacle Protein  International Journal of Molecular Sciences  2021  22:1240  PubMed ID: 33513895
16877  Akshat Joshi, Zhe Xu, Yasuhiro Ikegami, Kozue Yoshida, Yusuke Sakai, Akshay Joshi, Tejinder Kaur, Yosuke Nakao, Yo-ichi Yamashita, Hideo Baba, Shinichi Aishima, Neetu Singh, Hiroyuki Ijima  Exploiting synergistic effect of externally loaded bFGF and en  Chemical Engineering Journal  2021  404:126518 
11062  Rahman Md Moshikur, Md Raihan Chowdhury, Rie Wakabayashi, Yoshiro Tahara, Noriho Kamiya, Muhamma Moniruzzaman, Masahiro Goto  Ionic liquids with N-methyl-2-pyrrolidonium cation as an enhancer for topical drug delivery: Synthesis, characterization, and skin-penetration evaluation  Journal of Molecular Liquids  2020  299:112166 
13119  Enomoto U, Imashiro C, Takemura K.  Collective cell migration of fibroblasts is affected by horizontal vibration of the cell culture dish.  Eng Life Sci  2020    PubMed ID: 32944015
13445  Rahman Md Moshikur, Md. KorbanAli, Rie Wakabayashi, Muhammad Moniruzzaman, Masahiro Goto  Formation and potential application of micelles composed of biocompatible N-lauroyl-amino acid ionic liquids surfactant  Journal of Molecular Liquids  2020   
13855  Chikahiro Imashiro et al  Direct Cell Counting Using Macro-Scale Smartphone Images of Cell Aggregates  IEEE Access  2020  8 
11701  Yoshiki Fukuhara, Takayuki Takei, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida  Injectable Sugar Beet Pectin/Chitosan Derivative Composite Hydrogel for Wound Care  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN  2019   
12501  Sasaki JI, Katata C, Abe GL, Matsumoto T, Imazato S.  Fabricating large-scale three-dimensional constructs with living cells by processing with syringe needles  J Biomed Mater Res A  2019  107(4):904-909  PubMed ID: 30663860
10704  Hanagata N, Li X, Chen MH, Li J, Hattori S.  Double-stranded phosphodiester cytosine-guanine oligodeoxynucleotide complexed with calcium phosphate as a potent vaccine adjuvant for activating cellular and Th1-type humoral immunities.  Int J Nanomedicine  2017  13:43-62  PubMed ID: 29317815
12859  Hashimoto M, Yamaguchi S, Sasaki J, Kawai K, Kawakami H, Iwasaki Y, Imazato S.  Inhibition of matrix metalloproteinases and toxicity of gold and platinum nanoparticles in L929 fibroblast cells  Eur J Oral Sci  2016  124(1):68-74  PubMed ID: 26715398
12323  Lin X, Fukazawa K, Ishihara K.  Photoinduced inhibition of DNA unwinding in vitro with water-soluble polymers containing both phosphorylcholine and photoreactive groups  Acta Biomater  2016  40:226-234  PubMed ID: 27045692
14078  Shin-ya Furukawa, Gaku Hattori, Shinji Sakai, Noriho Kamiya  Highly efficient and low toxic skin penetrants composed of amino acid ionic liquids  RSC Advances  2016  6:87753-87755 
14998  Kousuke Moriyama, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya  Characterization of enzymatically gellable, phenolated linear poly(ethylene glycol) with different molecular weights for encapsulating living cells  Biochemical Engineering Journal  2015  93:25-30 
11037  Takemura R, Takaki H, Okada S, Shime H, Akazawa T, Oshiumi H, Matsumoto M, Teshima T, Seya T.  PolyI:C-Induced, TLR3/RIP3-Dependent Necroptosis Backs Up Immune Effector-Mediated Tumor Elimination In Vivo  Cancer Immunol Res  2015  3(8):902-14  PubMed ID: 25898986
15533  Masahito Miki, Masafumi Morita  Evaluation of the Biocompatibility of a Ti-Ta-Sn Alloy Using Cell Cultures.  Materials Transactions  2015  56:1087-109 
15984  Terada D, Hattori S, Honda T, Iitake M, Kobayashi H.  Embossed-carving processing of cytoskeletons of cultured cells by using focused ion beam technology  Microsc Res Tech  2013  76(3):290-5  PubMed ID: 23303728
7662  Byambaa B, Konno T, Ishihara K.  Cell adhesion control on photoreactive phospholipid polymer surfaces.  Colloids Surf B Biointerfaces  2011  99:1-6  PubMed ID: 21982212
8038  Sakai S, Kawakami K.  Development of porous alginate-based scaffolds covalently cross-linked through a peroxidase-catalyzed reaction.  J Biomater Sci Polym Ed  2011  22:2407-16  PubMed ID: 21144141
8202  Sakai S, Moriyama K, Kawakami K.  Controlling thermo-reversibility of gelatin gels through a peroxidase-catalyzed reaction under mild conditions for mammalian cells.  J Biomater Sci Polym Ed  2011  22:1147-56  PubMed ID: 20615328
8163  Sakai S, Moriyama K, Taguchi K, Kawakami K.  Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo.  Biomacromolecules  2010  11:2179-83  PubMed ID: 20690724
8287  Sakai S, Matsuyama T, Hirose K, Kawakami K.  In situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase.  Biomacromolecules  2010  11:1370-5  PubMed ID: 20402496
8477  Sakai S, Hirose K, Moriyama K, Kawakami K.  Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H(2)O(2) concentrations during gelation.  Acta Biomater  2010  6:1446-52  PubMed ID: 19818883
8521  Yamazoe H, Okuyama T, Suzuki H, Fukuda J.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.  Acta Biomater  2010  6:526-33  PubMed ID: 19654059
16937  Aya Saito, Tomohiro Konno, Hiroki Ikake, Kimio Kurita, Kazuhiko Ishihara  Glycoprotein recognition for cell capturing on artificial lectin surface  Transactions of the Materials Research Society of Japan  2010  35:127-130 
8612  Sakai S, Hirose K, Taguchi K, Ogushi Y, Kawakami K.  An injectable, in situ enzymatically gellable, gelatin derivative for drug delivery and tissue engineering.  Biomaterials  2009  30:3371-7  PubMed ID: 19345991
8709  Sakai S, Ogushi Y, Kawakami K.  Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: cellular adhesiveness and feasibility for cell sheet technology.  Acta Biomater  2009  5:554-9  PubMed ID: 19010747
6285  Nederberg F, Watanabe J, Ishihara K, Hilborn J, Bowden T.  Biocompatible and biodegradable phosphorylcholine ionomers with reduced protein adsorption and cell adhesion.  J Biomater Sci Polym Ed  2006  17(6):605-14  PubMed ID: 16892723
9029  Watanabe J, Ishihara K.  Cell engineering biointerface focusing on cytocompatibility using phospholipid polymer with an isomeric oligo(lactic acid) segment.  Biomacromolecules  2005  6:1797-802  PubMed ID: 15877407
16075  A. Oyane, T. Ishizone, M. Uchida, K. Furukawa, T. Ushida, H. Yokoyama  Spontaneous Formation of Blood-Compatible Surfaces on Hydrophobic Polymers: Surface Enrichment of a Block Copolymer with a Water-Soluble Block  Advanced Materials  2005  17:2329-2332 

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