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Cell No. : Cell Name
RCB1451 : L929  update : 2018/08/01
CommentSanford's original, brought by Katsuta at 7/5/1955. Insensitive to TNFalpha. Possible to culture in MEM medium.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Year of deposit 1998
Animal mouse < Mammals
Genus Mus
Species musculus
Tissue connective tissue
Classification other
History RCB0081
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 5% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 13

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537  Stanley ER, Heard PM.  Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.  J Biol Chem  1977  252(12):4305-12  PubMed ID: 301140
4661  WAYMOUTH C.  Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).  J Natl Cancer Inst  1959  22(5):1003-17  PubMed ID: 13655076
1817  SANFORD KK, HOBBS GL, EARLE WR.  The tumor-producing capacity of strain L mouse cells after 10 years in vitro.  Cancer Res  1956  16(2):162-6  PubMed ID: 13293658
4660  EAGLE H.  The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.  J Exp Med  1955  102(5):595-600  PubMed ID: 13271674
1818  EARLE WR, SCHILLING EL, BRYANT JC, EVANS VJ.  The growth of pure strain L cells in fluid-suspension cultures.  J Natl Cancer Inst.  1954  14(5):1159-71  PubMed ID: 13233852
4659  EVANS VJ, SHANNON JE Jr, BRYANT JC, WALTZ HK, EARLE WR, SANFORD KK.  A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.  J Natl Cancer Inst  1953  13(4):773-84  PubMed ID: 13035407
4658  SANFORD KK, EARLE WR, LIKELY GD.  The growth in vitro of single isolated tissue cells.  J Natl Cancer Inst  1948  9(3):229-46  PubMed ID: 18105872
4657  Earle, W.R  Production of malignancy in vitro.Ⅳ.The mouse fibroblast cultures and changes seen in the living cells.  J. Nat. Cancer Inst.  1943  4:165-212 

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User's Publication
11701  Yoshiki Fukuhara, Takayuki Takei, Takuma Yoshinaga, Hiroto Nishimata, Masahiro Yoshida  Injectable Sugar Beet Pectin/Chitosan Derivative Composite Hydrogel for Wound Care  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN  2019   
10704  Hanagata N, Li X, Chen MH, Li J, Hattori S.  Double-stranded phosphodiester cytosine-guanine oligodeoxynucleotide complexed with calcium phosphate as a potent vaccine adjuvant for activating cellular and Th1-type humoral immunities.  Int J Nanomedicine  2018  13:43-62  PubMed ID: 29317815
7662  Byambaa B, Konno T, Ishihara K.  Cell adhesion control on photoreactive phospholipid polymer surfaces.  Colloids Surf B Biointerfaces  2011  99:1-6  PubMed ID: 21982212
8038  Sakai S, Kawakami K.  Development of porous alginate-based scaffolds covalently cross-linked through a peroxidase-catalyzed reaction.  J Biomater Sci Polym Ed  2010  22:2407-16  PubMed ID: 21144141
8163  Sakai S, Moriyama K, Taguchi K, Kawakami K.  Hematin is an alternative catalyst to horseradish peroxidase for in situ hydrogelation of polymers with phenolic hydroxyl groups in vivo.  Biomacromolecules  2010  11:2179-83  PubMed ID: 20690724
8202  Sakai S, Moriyama K, Kawakami K.  Controlling thermo-reversibility of gelatin gels through a peroxidase-catalyzed reaction under mild conditions for mammalian cells.  J Biomater Sci Polym Ed  2010  22:1147-56  PubMed ID: 20615328
8287  Sakai S, Matsuyama T, Hirose K, Kawakami K.  In situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase.  Biomacromolecules  2010  11:1370-5  PubMed ID: 20402496
8477  Sakai S, Hirose K, Moriyama K, Kawakami K.  Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H(2)O(2) concentrations during gelation.  Acta Biomater  2009  6:1446-52  PubMed ID: 19818883
8521  Yamazoe H, Okuyama T, Suzuki H, Fukuda J.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.  Acta Biomater  2009  6:526-33  PubMed ID: 19654059
8612  Sakai S, Hirose K, Taguchi K, Ogushi Y, Kawakami K.  An injectable, in situ enzymatically gellable, gelatin derivative for drug delivery and tissue engineering.  Biomaterials  2009  30:3371-7  PubMed ID: 19345991
8709  Sakai S, Ogushi Y, Kawakami K.  Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: cellular adhesiveness and feasibility for cell sheet technology.  Acta Biomater  2008  5:554-9  PubMed ID: 19010747
6285  Nederberg F, Watanabe J, Ishihara K, Hilborn J, Bowden T.  Biocompatible and biodegradable phosphorylcholine ionomers with reduced protein adsorption and cell adhesion.  J Biomater Sci Polym Ed  2006  17(6):605-14  PubMed ID: 16892723
9029  Watanabe J, Ishihara K.  Cell engineering biointerface focusing on cytocompatibility using phospholipid polymer with an isomeric oligo(lactic acid) segment.  Biomacromolecules  2005  6:1797-802  PubMed ID: 15877407

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