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Cell No. : Cell Name
RCB1450 : LM8  update : 2022/04/01
CommentA highly metastatic cell line derived from Dunn's osteosarcoma (C3H mouse origin).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ueda, Takafumi
Originator Aoki, Yasuaki & Ueda, Ueda, Takafumi
Year of deposit 1998
Animal mouse < Mammals
Strain name C3H
Gender Female
Tissue bone
Disease name osteosarcoma
Metastatic ability Yes
Metastatic tissue lung
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6669
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, MPI, PepB
Images
deposit info
lot info
Reference information Reference 2
User's Publication 52


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Reference
2332  Asai T, Ueda T, Itoh K, Yoshioka K, Aoki Y, Mori S, Yoshikawa H  Establishment and characterization of a murine osteosarcoma cell line (LM8) with high metastatic potential to the lung.  Int J Cancer  1998  76:418-22  PubMed ID: 9579581
2333  Mori S, Ueda T, Kuratsu S, Hosono N, Izawa K, Uchida A  Suppression of pulmonary metastasis by angiogenesis inhibitor TNP-470 in murine osteosarcoma.  Int J Cancer  1995  61:148-52  PubMed ID: 7535752

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User's Publication
16215  Manami Suzuki-Karasaki, Takashi Ando, Yushi Ochiai, Kenta Kawahara, Miki Suzuki-Karasaki, Hideki Nakayama, Yoshihiro Suzuki-Karasaki  Air Plasma-Activated Medium Evokes a Death-Associated Perinuclear Mitochondrial Clustering  Int J Mol Sci  2022  23(3):1124  PubMed ID: 35163042
16237  Inoue M, Horiuchi K, Susa M, Taguchi E, Ishizaka T, Rikitake H, Matsuhashi Y, Chiba K.  Trabectedin suppresses osteosarcoma pulmonary metastasis in a mouse tumor xenograft model  J Orthop Res  2022  May 31  PubMed ID: 34057747
20656  Ogiwara Y, Nakagawa M, Nakatani F, Uemura Y, Zhang R, Kudo-Saito C.  Blocking FSTL1 boosts NK immunity in treatment of osteosarcoma.  Cancer Lett  2022  537:215690  PubMed ID: 35439537
20032  Lin L, Wang H, Guo W, He E, Huang K, Zhao Q.  Osteosarcoma-derived exosomal miR-501-3p promotes osteoclastogenesis and aggravates bone loss.  Cell Signal  2021  82:109935  PubMed ID: 33529755
13630  Hennessy M, Wahba A, Felix K, Cabrera M, Segura MG, Kundra V, Ravoori MK, Stewart J, Kleinerman ES, Jensen VB, Gopalakrishnan V, Pena R, Quach P, Kim G, Kivimäe S, Madakamutil L, Overwijk WW, Zalevsky J, Gordon N.  Bempegaldesleukin (BEMPEG; NKTR-214) efficacy as a single agent and in combination with checkpoint-inhibitor therapy in mouse models of osteosarcoma  Int J Cancer  2021  148(8):1928-1937  PubMed ID: 33152115
14340  Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.  CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment  Mol Cancer Ther  2021    PubMed ID: 34088832
14344  Araki Y, Aiba H, Yoshida T, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Nguyen TD, Ishii KA, Nojima T, Takahashi S, Murakami H, Tsuchiya H, Hanayama R.  Osteosarcoma-Derived Small Extracellular Vesicles Enhance Tumor Metastasis and Suppress Osteoclastogenesis by miR-146a-5p  Front Oncol  2021  11:667109.  PubMed ID: 34017686
15140  Matsusaka K, Fujiwara Y, Pan C, Esumi S, Saito Y, Bi J, Nakamura Y, Mukunoki A, Takeo T, Nakagata N, Yoshii D, Fukuda R, Nagasaki T, Tanaka R, Komori H, Maeda H, Watanabe H, Tamada K, Komohara Y, Maruyama T.  α1-acid glycoprotein enhances the immunosuppressive and protumor functions of tumor-associated macrophages  Cancer Res  2021  canres.3471.2020  PubMed ID: 34210751
20267  Maruyama K, Okada T, Ueha T, Isohashi K, Ikeda H, Kanai Y, Sasaki K, Gentsu T, Ueshima E, Sofue K, Nogami M, Yamaguchi M, Sugimoto K, Sakai Y, Hatazawa J, Murakami T.  In vivo evaluation of percutaneous carbon dioxide treatment for improving intratumoral hypoxia using 18F-fluoromisonidazole PET-CT.  Oncol Lett  2021  21(3):207  PubMed ID: 33574946
20272  Ma Y, Yoshida T, Matoba K, Kida K, Shintani R, Piao Y, Jin J, Nishino T, Hanayama R.  Identification of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA.  Sci Rep  2021  11(1):13471  PubMed ID: 34188113
16104  Kinoshita H, Shimozato O, Ishii T, Kamoda H, Hagiwara Y, Tsukanishi T, Ohtori S, Yonemoto T.  The Thioredoxin Reductase Inhibitor Auranofin Suppresses Pulmonary Metastasis of Osteosarcoma, But Not Local Progression  Anticancer Res  2021  41(10):4947-4955  PubMed ID: 34593442
16109  Kinoshita H, Shimozato O, Ishii T, Kamoda H, Hagiwara Y, Ohtori S, Yonemoto T.  The Thioredoxin-1 Inhibitor, PX-12, Suppresses Local Osteosarcoma Progression  Anticancer Res  2021  41(12):6013-6021  PubMed ID: 34848455
16389  Ideta H, Yoshida K, Okamoto M, Sasaki J, Kito M, Aoki K, Yoshimura Y, Suzuki S, Tanaka A, Takazawa A, Haniu H, Uemura T, Takizawa T, Sobajima A, Kamanaka T, Takahashi J, Kato H, Saito N.  Antitumor Effect of Sclerostin against Osteosarcoma  Cancers (Basel)  2021  13(23):6015  PubMed ID: 34885123
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969
12049  Yahiro K, Matsumoto Y, Yamada H, Endo M, Setsu N, Fujiwara T, Nakagawa M, Kimura A, Shimada E, Okada S, Oda Y, Nakashima Y.  Activation of TLR4 signaling inhibits progression of osteosarcoma by stimulating CD8-positive cytotoxic lymphocytes.  Cancer Immunol. Immunother.  2020    PubMed ID: 32047957
13305  Wang X, Xue J, Ma B, Wu J, Chang J, Gelinsky M, Wu C.  Black Bioceramics: Combining Regeneration with Therapy.  Adv Mater  2020    PubMed ID: 33094493
13306  Ando T, Ichikawa J, Fujimaki T, Taniguchi N, Takayama Y, Haro H.  Gemcitabine and Rapamycin Exhibit Additive Effect against Osteosarcoma by Targeting Autophagy and Apoptosis.  Cancers (Basel)  2020    PubMed ID: 33114161
13310  Sakuda T, Kubo T, Johan MP, Furuta T, Sakaguchi T, Adachi N.  Development of an Oncolytic Recombinant Vesicular Stomatitis Virus Encoding a Tumor-suppressor MicroRNA.  Anticancer Res  2020    PubMed ID: 33109569
13527  Takashi Ando, Manami Suzuki-Karasaki, Miki Suzuki-Karasaki, Jiro Ichikawa, Toyoko Ochiai, Yukihiro Yoshida, Hirotaka Haro, Yoshihiro Suzuki-Karasaki  Synergistic anticancer effect of plasma-activated infusion and salinomycin by targeting autophagy and mitochondrial morphology  bioRxiv  2020  October 15 
13532  Pan C, Fujiwara Y, Horlad H, Shiraishi D, Iriki T, Tsuboki J, Ikeda T, Komohara Y.  Flavonoid Compounds Contained in Epimedii Herba Inhibit Tumor Progression by Suppressing STAT3 Activation in the Tumor Microenvironment  Front Pharmacol  2020  11:262  PubMed ID: 32256354
14872  Ma L, Sakamoto Y, Kanai A, Otsuka H, Takahashi A, Kakimi K, Imai T, Shimokawa T.  Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by In Vivo Selection Method  J Mol Sci  2020  21(8):2829  PubMed ID: 32325684
15141  Takenaka W, Takahashi Y, Tamari K, Minami K, Katsuki S, Seo Y, Isohashi F, Koizumi M, Ogawa K.  Radiation Dose Escalation is Crucial in Anti-CTLA-4 Antibody Therapy to Enhance Local and Distant Antitumor Effect in Murine Osteosarcoma  Cancers (Basel)  2020  12(6):1546  PubMed ID: 32545427
12504  Takahashi Y, Yasui T, Minami K, Tamari K, Hayashi K, Otani K, Seo Y, Isohashi F, Koizumi M, Ogawa K.  Carbon ion irradiation enhances the antitumor efficacy of dual immune checkpoint blockade therapy both for local and distant sites in murine osteosarcoma  Oncotarget  2019  10(6):633-646  PubMed ID: 30774761
12505  Sakuda T, Kubo T, Johan MP, Furuta T, Sakaguchi T, Nakanishi M, Ochi M, Adachi N.  Novel Near-Infrared Fluorescence-Guided Surgery With Vesicular Stomatitis Virus for Complete Surgical Resection of Osteosarcomas in Mice  J Orthop Res  2019  37(5):1192-1201  PubMed ID: 30839125
11337  Takahashi A, Wakihata S, Ma L, Adachi T, Hirose H, Yoshida Y, Ohira Y.  Temporary Loading Prevents Cancer Progression and Immune Organ Atrophy Induced by Hind-Limb Unloading in Mice.  Int J Mol Sci  2018    PubMed ID: 30544854
4277  Shimizu T, Fuchimoto Y, Okita H, Fukuda K, Kitagawa Y, Ueno S, Kuroda T.  A curative treatment strategy using tumor debulking surgery combined with immune checkpoint inhibitors for advanced pediatric solid tumors: An in vivo study using a murine model of osteosarcoma.  J. Pediatr. Surg.  2018    PubMed ID: 30266483
4278  Johan MP, Kubo T, Furuta T, Sakuda T, Sakaguchi T, Nakanishi M, Ochi M, Adachi N.  Metastatic tumor cells detection and anti-metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma.  J. Orthop. Res.  2018    PubMed ID: 29637599
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427
14821  Shiraishi D, Fujiwara Y, Horlad H, Saito Y, Iriki T, Tsuboki J, Cheng P, Nakagata N, Mizuta H, Bekki H, Nakashima Y, Oda Y, Takeya M, Komohara Y.  CD163 Is Required for Protumoral Activation of Macrophages in Human and Murine Sarcoma  Cancer Res  2018  78(12):3255-3266  PubMed ID: 29610117
10239  Kidani T, Yasuda R, Miyawaki J, Oshima Y, Miura H, Masuno H.  Bisphenol A Inhibits Cell Proliferation and Reduces the Motile Potential of Murine LM8 Osteosarcoma Cells.  Anticancer Res.  2017  37:1711-1722  PubMed ID: 28373433
10496  Shimizu T, Fuchimoto Y, Fukuda K, Okita H, Kitagawa Y, Kuroda T.  The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma.  J. Pediatr. Surg.  2017  52:2047-2050  PubMed ID: 28954696
14705  Tsuda Y, Tanikawa C, Miyamoto T, Hirata M, Yodsurang V, Zhang YZ, Imoto S, Yamaguchi R, Miyano S, Takayanagi H, Kawano H, Nakagawa H, Tanaka S, Matsuda K.  Identification of a p53 target, CD137L, that mediates growth suppression and immune response of osteosarcoma cells  Sci Rep  2017  7(1):10739  PubMed ID: 28878391
14753  Takahashi Y, Yasui T, Minami K, Tamari K, Otani K, Seo Y, Isohashi F, Koizumi M, Ogawa K.  Radiation Enhances the Efficacy of Antitumor Immunotherapy with an Immunocomplex of Interleukin-2 and Its Monoclonal Antibody  Anticancer Res  2017  37(12):6799-6806  PubMed ID: 29187458
9693  Xing Y, Tian Y, Kurosawa T, Matsui S, Touma M, Yanai T, Wu Q, Sugimoto K.  CCL11-induced eosinophils inhibit the formation of blood vessels and cause tumor necrosis.  Genes Cells  2016  21:624-38  PubMed ID: 27169545
9750  Fujiwara Y, Horlad H, Shiraishi D, Tsuboki J, Kudo R, Ikeda T, Nohara T, Takeya M, Komohara Y.  Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.  Mol Nutr Food Res  2016  60:2467-2480  PubMed ID: 27393711
9832  Yang D, Okamura H, Morimoto H, Teramachi J, Haneji T.  Protein phosphatase 2A Cα regulates proliferation, migration, and metastasis of osteosarcoma cells.  Lab. Invest.  2016  96:1050-62  PubMed ID: 27617401
10853  Kazuyuki Itoh  Basic Research for Osteosarcoma Lung Metastasis (LM8)  Osteosarcoma  2016  19-29 
12675  Dovedi SJ, Adlard AL, Ota Y, Murata M, Sugaru E, Koga-Yamakawa E, Eguchi K, Hirose Y, Yamamoto S, Umehara H, Honeychurch J, Cheadle EJ, Hughes G, Jewsbury PJ, Wilkinson RW, Stratford IJ, Illidge TM.  Intravenous administration of the selective toll-like receptor 7 agonist DSR-29133 leads to anti-tumor efficacy in murine solid tumor models which can be potentiated by combination with fractionated radiotherapy  Oncotarget  2016  7(13):17035-46  PubMed ID: 26959743
12971  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H.  Dendritic cells combined with doxorubicin induces immunogenic cell death and exhibits antitumor effects for osteosarcoma  Oncol Lett   2016  11(3):2169-2175  PubMed ID: 26998143
7121  Kato S, Mori S, Kodama T.  A Novel Treatment Method for Lymph Node Metastasis Using a Lymphatic Drug Delivery System with Nano/Microbubbles and Ultrasound.  J Cancer  2015  6:1282-94  PubMed ID: 26640589
7258  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H.  Dendritic cells combined with anti-GITR antibody produce antitumor effects in osteosarcoma.  Oncol. Rep.  2015  34:1995-2001  PubMed ID: 26239052
7502  Nakata H, Miyazaki T, Iwasaki T, Nakamura A, Kidani T, Sakayama K, Masumoto J, Miura H.  Development of tumor-specific caffeine-potentiated chemotherapy using a novel drug delivery system with Span 80 nano-vesicles.  Oncol. Rep.  2015  33:1593-8  PubMed ID: 25633802
7528  Fujiwara Y, Shiraishi D, Yoshitomi M, Ikeda T, Mizuta H, Takeya M, Komohara Y  Soyasapogenols contained in soybeans suppress tumour progression by regulating macrophage differentiation into the protumoural phenotype.  J Functional Foods  2015  19:594-605 
15438  Igarashi K, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Odani A, Tsuchiya H.  Effectiveness of two novel anionic and cationic platinum complexes in the treatment of osteosarcoma  Anticancer Agents Med Chem   2015  15(3):390-9  PubMed ID: 25511510
7854  Zhang D, Pan X, Ohno S, Osuga T, Sawada S, Sato K.  No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase-2/9 in osteosarcoma cell lines.  Bioelectromagnetics  2011  32:463-73  PubMed ID: 21480303
7959  Tsubaki M, Yamazoe Y, Yanae M, Satou T, Itoh T, Kaneko J, Kidera Y, Moriyama K, Nishida S.  Blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathways by statins reduces the expression of bFGF, HGF, and TGF-β as angiogenic factors in mouse osteosarcoma.  Cytokine  2011  54:100-7  PubMed ID: 21292498
8036  Nakazawa Y, Arai H, Fujita N.  The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis.  Cancer Res.  2011  71:1146-55  PubMed ID: 21148752
5862  Rojas A, González I, Rodríguez B, Romero J, Figueroa H, Llanos J, Morales E, Pérez-Castro R.  Evidence of involvement of the receptor for advanced glycation end-products (RAGE) in the adhesion of Helicobacter pylori to gastric epithelial cells.  Microbes Infect  2011  Sep;13(10):818-23  PubMed ID: 21609778
8334  Kawano M, Nishida H, Nakamoto Y, Tsumura H, Tsuchiya H.  Cryoimmunologic antitumor effects enhanced by dendritic cells in osteosarcoma.  Clin. Orthop. Relat. Res.  2010  468:1373-83  PubMed ID: 20232181
8693  Kamei S, Sakayama K, Tamashiro S, Aizawa J, Miyawaki J, Miyazaki T, Yamamoto H, Norimatsu Y, Masuno H.  Ketoprofen in topical formulation decreases the matrix metalloproteinase-2 expression and pulmonary metastatic incidence in nude mice with osteosarcoma.  J. Orthop. Res.  2008  27:909-15  PubMed ID: 19105229
3384  Ando, Takashi, Ichikawa, Jiro, Okamoto, Atsushi, Tasaka, Kachio, Nakao, Atsuhito, Hamada, Yoshiki  Gemcitabine inhibits viability, growth, and metastasis of osteosarcoma cell lines.  J Orthop Res  2005  23:964-9  PubMed ID: 16023013



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