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Cell No. : Cell Name
RCB1450 : LM8  update : 2020/09/24
CommentA highly metastatic cell line derived from Dunn's osteosarcoma (C3H mouse origin).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ueda, Takafumi
Originator Aoki, Yasuaki & Ueda, Ueda, Takafumi
Year of deposit 1998
Animal mouse < Mammals
Strain name C3H
Gender Female
Tissue bone
Case history osteosarcoma
Metastastatic ability Yes
Metastatsd tissue lung
Classification cancer
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, MPI, PepB
deposit info
lot info
Reference information Reference 2
User's Publication 22

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2332  Asai T, Ueda T, Itoh K, Yoshioka K, Aoki Y, Mori S, Yoshikawa H  Establishment and characterization of a murine osteosarcoma cell line (LM8) with high metastatic potential to the lung.  Int J Cancer  1998  76:418-22  PubMed ID: 9579581
2333  Mori S, Ueda T, Kuratsu S, Hosono N, Izawa K, Uchida A  Suppression of pulmonary metastasis by angiogenesis inhibitor TNP-470 in murine osteosarcoma.  Int J Cancer  1995  61:148-52  PubMed ID: 7535752

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User's Publication
11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020    PubMed ID: 31914969
12049  Yahiro K, Matsumoto Y, Yamada H, Endo M, Setsu N, Fujiwara T, Nakagawa M, Kimura A, Shimada E, Okada S, Oda Y, Nakashima Y.  Activation of TLR4 signaling inhibits progression of osteosarcoma by stimulating CD8-positive cytotoxic lymphocytes.  Cancer Immunol. Immunother.  2020    PubMed ID: 32047957
11337  Takahashi A, Wakihata S, Ma L, Adachi T, Hirose H, Yoshida Y, Ohira Y.  Temporary Loading Prevents Cancer Progression and Immune Organ Atrophy Induced by Hind-Limb Unloading in Mice.  Int J Mol Sci  2018    PubMed ID: 30544854
4277  Shimizu T, Fuchimoto Y, Okita H, Fukuda K, Kitagawa Y, Ueno S, Kuroda T.  A curative treatment strategy using tumor debulking surgery combined with immune checkpoint inhibitors for advanced pediatric solid tumors: An in vivo study using a murine model of osteosarcoma.  J. Pediatr. Surg.  2018    PubMed ID: 30266483
4278  Johan MP, Kubo T, Furuta T, Sakuda T, Sakaguchi T, Nakanishi M, Ochi M, Adachi N.  Metastatic tumor cells detection and anti-metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma.  J. Orthop. Res.  2018    PubMed ID: 29637599
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427
10239  Kidani T, Yasuda R, Miyawaki J, Oshima Y, Miura H, Masuno H.  Bisphenol A Inhibits Cell Proliferation and Reduces the Motile Potential of Murine LM8 Osteosarcoma Cells.  Anticancer Res.  2017  37:1711-1722  PubMed ID: 28373433
10496  Shimizu T, Fuchimoto Y, Fukuda K, Okita H, Kitagawa Y, Kuroda T.  The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma.  J. Pediatr. Surg.  2017  52:2047-2050  PubMed ID: 28954696
9693  Xing Y, Tian Y, Kurosawa T, Matsui S, Touma M, Yanai T, Wu Q, Sugimoto K.  CCL11-induced eosinophils inhibit the formation of blood vessels and cause tumor necrosis.  Genes Cells  2016  21:624-38  PubMed ID: 27169545
9750  Fujiwara Y, Horlad H, Shiraishi D, Tsuboki J, Kudo R, Ikeda T, Nohara T, Takeya M, Komohara Y.  Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.  Mol Nutr Food Res  2016  60:2467-2480  PubMed ID: 27393711
9832  Yang D, Okamura H, Morimoto H, Teramachi J, Haneji T.  Protein phosphatase 2A Cα regulates proliferation, migration, and metastasis of osteosarcoma cells.  Lab. Invest.  2016  96:1050-62  PubMed ID: 27617401
10853  Kazuyuki Itoh  Basic Research for Osteosarcoma Lung Metastasis (LM8)  Osteosarcoma  2016  19-29 
7258  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H.  Dendritic cells combined with anti-GITR antibody produce antitumor effects in osteosarcoma.  Oncol. Rep.  2015  34:1995-2001  PubMed ID: 26239052
7502  Nakata H, Miyazaki T, Iwasaki T, Nakamura A, Kidani T, Sakayama K, Masumoto J, Miura H.  Development of tumor-specific caffeine-potentiated chemotherapy using a novel drug delivery system with Span 80 nano-vesicles.  Oncol. Rep.  2015  33:1593-8  PubMed ID: 25633802
7528  Fujiwara Y, Shiraishi D, Yoshitomi M, Ikeda T, Mizuta H, Takeya M, Komohara Y  Soyasapogenols contained in soybeans suppress tumour progression by regulating macrophage differentiation into the protumoural phenotype.  J Functional Foods  2015  19:594-605 
7854  Zhang D, Pan X, Ohno S, Osuga T, Sawada S, Sato K.  No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase-2/9 in osteosarcoma cell lines.  Bioelectromagnetics  2011  32:463-73  PubMed ID: 21480303
7959  Tsubaki M, Yamazoe Y, Yanae M, Satou T, Itoh T, Kaneko J, Kidera Y, Moriyama K, Nishida S.  Blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathways by statins reduces the expression of bFGF, HGF, and TGF-β as angiogenic factors in mouse osteosarcoma.  Cytokine  2011  54:100-7  PubMed ID: 21292498
5862  Rojas A, González I, Rodríguez B, Romero J, Figueroa H, Llanos J, Morales E, Pérez-Castro R.  Evidence of involvement of the receptor for advanced glycation end-products (RAGE) in the adhesion of Helicobacter pylori to gastric epithelial cells.  Microbes Infect  2011  Sep;13(10):818-23  PubMed ID: 21609778
8036  Nakazawa Y, Arai H, Fujita N.  The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis.  Cancer Res.  2010  71:1146-55  PubMed ID: 21148752
8334  Kawano M, Nishida H, Nakamoto Y, Tsumura H, Tsuchiya H.  Cryoimmunologic antitumor effects enhanced by dendritic cells in osteosarcoma.  Clin. Orthop. Relat. Res.  2010  468:1373-83  PubMed ID: 20232181
8693  Kamei S, Sakayama K, Tamashiro S, Aizawa J, Miyawaki J, Miyazaki T, Yamamoto H, Norimatsu Y, Masuno H.  Ketoprofen in topical formulation decreases the matrix metalloproteinase-2 expression and pulmonary metastatic incidence in nude mice with osteosarcoma.  J. Orthop. Res.  2008  27:909-15  PubMed ID: 19105229
3384  Ando, Takashi, Ichikawa, Jiro, Okamoto, Atsushi, Tasaka, Kachio, Nakao, Atsuhito, Hamada, Yoshiki  Gemcitabine inhibits viability, growth, and metastasis of osteosarcoma cell lines.  J Orthop Res  2005  23:964-9  PubMed ID: 16023013

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