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Cell No. : Cell Name
RCB1449 : C7  update : 2023/09/27
Commentp53 deficient mouse bone marrow derived cell. Useful for study of p53 suppressor oncogene.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hayashi, Shin-ichi
Originator Hayashi, Shin-ichi
Year of deposit 1998
Animal _mouse < Mammals
Strain name 129/sv-(p53-/-)
Gender Male
Age at sampling 3 months
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene p53(-/-), Neo
Year of origin 1996
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_C118
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS + 0.5ng/ml M-CSF
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
deposit info
lot info
Reference information Reference 1
User's Publication 0

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2676  Miyamoto A, Kunisada T, Hemmi H, Yamane T, Yasuda H, Miyake K, Yamazaki H, Hayashi, S I  Establishment and characterization of an immortal macrophage-like cell line inducible to differentiate to osteoclasts.  Biochem Biophys Res Commun  1998  242:703-9  PubMed ID: 9464281   DOI: 10.1006/bbrc.1997.8046

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