細胞番号 : 細胞名
RCB1435 : KE-97
update : 2024/01/17
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細胞特性(Comment:英) | Stopped distribution. Until 2022, this cell line has been distributed as “Human cell line derived from intraperitoneal metastasis of gastric cancer”. However, this cell line was proved to be derived from B cells, and thus we stopped the distribution. |
細胞特性(日) | 提供中止。2022年まで、「ヒト胃癌の腹腔内(腸間膜)転移巣に由来する細胞株」と表示して提供していたが、B細胞由来の細胞株であることが分かり、提供を中止した。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | There is no restriction regarding use for basic researches. Commercial use is prohibited by the DEPOSITOR. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業目的の利用不可。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
Uesugi, Hidenaga
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樹立者 |
Yamashita, Kazuya
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寄託日 |
1998
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動物種 |
_human
< Mammals
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属名 |
Homo
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種名 |
sapiens
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人種 |
Japanese
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性別 |
Male
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齢 |
52 years
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採取組織 |
stomach, mesenterium meta
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細胞分類 |
cancer
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初代培養日 |
1989
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細胞寿命 |
infinite
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細胞形態 |
other
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Cellosaurus(Expasy) |
CVCL_3386
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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培地 |
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RPMI1640 + 10% FBS
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抗生物質 |
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Free
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継代方法 |
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pipetting
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継代密度 |
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1 : 8 split
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継代・培地交換頻度 |
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Subculture : 2 times/week
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培養最適温度 |
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37
℃
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二酸化炭素濃度 |
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5
%
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凍結培地 |
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Medium + 10% DMSO
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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アイソザイム検査 |
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LD, NP
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染色体検査 |
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46-48(50) : 46(3),47(41),48(6)
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個体識別検査 |
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OK
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画像情報 |
寄託時情報 | ロット情報 |
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文献情報 |
Reference(英) |
1件
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Reference(日) |
1件
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利用者成果(英) |
11件
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利用者成果(日) |
0件
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利用者成果(英) |
16129
Nagamura Y, Miyazaki M, Nagano Y, Tomiyama A, Ohki R, Yanagihara K, Sakai R, Yamaguchi H.
SHP2 as a Potential Therapeutic Target in Diffuse-Type Gastric Carcinoma Addicted to Receptor Tyrosine Kinase Signaling
Cancers (Basel)
2021
13(17):4309
PubMed ID: 34503119
DOI: 10.3390/cancers13174309
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11505
Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.
Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.
Biochem Biophys Rep
2019
18:100632
PubMed ID: 30993217
DOI: 10.1016/j.bbrep.2019.100632
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10662
Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.
Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.
Biochem. Biophys. Res. Commun.
2018
495:1942-1947
PubMed ID: 29247652
DOI: 10.1016/j.bbrc.2017.12.067
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10586
Ishii S, Yamashita K, Harada H, Ushiku H, Tanaka T, Nishizawa N, Yokoi K, Washio M, Ema A, Mieno H, Moriya H, Hosoda K, Waraya M, Katoh H, Watanabe M.
The H19-PEG10/IGF2BP3 axis promotes gastric cancer progression in patients with high lymph node ratios.
Oncotarget
2017
8:74567-74581
PubMed ID: 29088808
DOI: 10.18632/oncotarget.20209
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5812
Hirashita Y et al
Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.
Cancer Sci
2016
107(12):1919-1928
PubMed ID: 27699948
DOI: 10.1111/cas.13094
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12639
Yamashita K, Kuno A, Matsuda A, Ikehata Y, Katada N, Hirabayashi J, Narimatsu H, Watanabe M.
Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer
Gastric Cancer
2016
19(2):531-54
PubMed ID: 25840959
DOI: 10.1007/s10120-015-0491-2
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5837
Liu J et al
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat Commun
2014
5:3830
PubMed ID: 24807215
DOI: 10.1038/ncomms4830
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5302
Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.
Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.
Cancer Sci
2014
105(5):528-36
PubMed ID: 24612061
DOI: 10.1111/cas.12387
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5845
Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.
Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.
PLoS One
2013
8(2):e56766
PubMed ID: 23451082
DOI: 10.1371/journal.pone.0056766
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15081
Awazu Y, Nakamura K, Mizutani A, Kakoi Y, Iwata H, Yamasaki S, Miyamoto N, Imamura S, Miki H, Hori A.
A novel inhibitor of c-Met and VEGF receptor tyrosine kinases with a broad spectrum of in vivo antitumor activities
Mol Cancer Ther
2013
12(6):913-24
PubMed ID: 23548264
DOI: 10.1158/1535-7163.MCT-12-1011
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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