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Cell No. : Cell Name
RCB1435 : KE-97  update : 2022/10/12
CommentFor precise analysis of characteristics, distribution is stopped temporarily. Human cell line derived from intraperitoneal metastasis of gastric cancer
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. Commercial use is prohibited by the DEPOSITOR.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Uesugi, Hidenaga
Originator Yamashita, Kazuya
Year of deposit 1998
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 52 years
Tissue stomach, mesenterium meta
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3386
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 46-48(50) : 46(3),47(41),48(6)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 11


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Reference
2692  Uesugi H, Atari E  [Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line]  Nippon Shokakibyo Gakkai Zasshi  1995  92:19-25  PubMed ID: 7861622  

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User's Publication
16129  Nagamura Y, Miyazaki M, Nagano Y, Tomiyama A, Ohki R, Yanagihara K, Sakai R, Yamaguchi H.  SHP2 as a Potential Therapeutic Target in Diffuse-Type Gastric Carcinoma Addicted to Receptor Tyrosine Kinase Signaling  Cancers (Basel)  2021  13(17):4309  PubMed ID: 34503119   DOI: 10.3390/cancers13174309
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632
10662  Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.  Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.  Biochem. Biophys. Res. Commun.  2018  495:1942-1947  PubMed ID: 29247652   DOI: 10.1016/j.bbrc.2017.12.067
10586  Ishii S, Yamashita K, Harada H, Ushiku H, Tanaka T, Nishizawa N, Yokoi K, Washio M, Ema A, Mieno H, Moriya H, Hosoda K, Waraya M, Katoh H, Watanabe M.  The H19-PEG10/IGF2BP3 axis promotes gastric cancer progression in patients with high lymph node ratios.  Oncotarget  2017  8:74567-74581  PubMed ID: 29088808   DOI: 10.18632/oncotarget.20209
5812  Hirashita Y et al  Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.  Cancer Sci  2016  107(12):1919-1928  PubMed ID: 27699948   DOI: 10.1111/cas.13094
12639  Yamashita K, Kuno A, Matsuda A, Ikehata Y, Katada N, Hirabayashi J, Narimatsu H, Watanabe M.  Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer  Gastric Cancer  2016  19(2):531-54  PubMed ID: 25840959   DOI: 10.1007/s10120-015-0491-2
5837  Liu J et al  Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.  Nat Commun  2014  5:3830  PubMed ID: 24807215   DOI: 10.1038/ncomms4830
5302  Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.  Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.  Cancer Sci  2014  105(5):528-36  PubMed ID: 24612061   DOI: 10.1111/cas.12387
5845  Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.  Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.  PLoS One  2013  8(2):e56766  PubMed ID: 23451082   DOI: 10.1371/journal.pone.0056766
15081  Awazu Y, Nakamura K, Mizutani A, Kakoi Y, Iwata H, Yamasaki S, Miyamoto N, Imamura S, Miki H, Hori A.  A novel inhibitor of c-Met and VEGF receptor tyrosine kinases with a broad spectrum of in vivo antitumor activities  Mol Cancer Ther  2013  12(6):913-24  PubMed ID: 23548264   DOI: 10.1158/1535-7163.MCT-12-1011
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x



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