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Cell No. : Cell Name
RCB1434 : KE-39  update : 2023/01/05
CommentHuman cell line derived from gastric cancer
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. Commercial use is prohibited by the DEPOSITOR.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Uesugi, Hidenaga
Originator Yamashita, Kazuya
Year of deposit 1998
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 77 years
Tissue stomach, lymph node meta
Disease name gastric cancer
Metastatic ability Yes
Classification cancer
Year of origin 1991
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3385
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (EBV) (+)
Isozyme LD, NP
Chromosome mode 59-65(50) : 59(3),60(4),61(8),62(20),63(11),64(3)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 8

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2691  Uesugi H, Yamashita K, Atari E, Shinoda H, Nishiyama T, Okayasu I, Yasuda S  [Establishment and characterization of strain KE-39 derived from human gastric cancer]  Hum Cell  1994  7:227-32  PubMed ID: 7599112  

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User's Publication
21415  Fukuoka S, Koga Y, Yamauchi M, Koganemaru S, Yasunaga M, Shitara K, Doi T, Yoshino T, Kuronita T, Elenbaas B, Wahra P, Zhang H, Crowley L, Jenkins MH, Clark A, Kojima T.  p70S6K/Akt dual inhibitor DIACC3010 is efficacious in preclinical models of gastric cancer alone and in combination with trastuzumab.  Sci Rep  2023  13(1):16017  PubMed ID: 37749105   DOI: 10.1038/s41598-023-40612-9
13911  Kawahata T, Kawahara K, Shimokawa M, Sakiyama A, Shiraishi T, Minami K, Yamamoto M, Shinsato Y, Arima K, Hamada T, Furukawa T.  Involvement of ribosomal protein L11 expression in sensitivity of gastric cancer against 5-FU  Oncol Lett  2020  19(3):2258-2264  PubMed ID: 32194724   DOI: 10.3892/ol.2020.11352
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632
14615  Nambara S, Masuda T, Nishio M, Kuramitsu S, Tobo T, Ogawa Y, Hu Q, Iguchi T, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Saeki H, Oki E, Maehara Y, Suzuki A, Mimori K.  Antitumor effects of the antiparasitic agent ivermectin via inhibition of Yes-associated protein 1 expression in gastric cancer  Oncotarget   2017  8(64):107666-107677  PubMed ID: 29296196   DOI: 10.18632/oncotarget.22587
5812  Hirashita Y et al  Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.  Cancer Sci  2016  107(12):1919-1928  PubMed ID: 27699948   DOI: 10.1111/cas.13094
5837  Liu J et al  Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.  Nat Commun  2014  5:3830  PubMed ID: 24807215   DOI: 10.1038/ncomms4830
5845  Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.  Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.  PLoS One  2013  8(2):e56766  PubMed ID: 23451082   DOI: 10.1371/journal.pone.0056766
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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