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Cell No. : Cell Name
RCB1434 : KE-39  update : 2020/09/24
CommentHuman cell line derived from gastric cancer
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Uesugi, Hidenaga
Originator Yamashita, Kazuya
Year of deposit 1998
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 77 years
Tissue stomach, lymph node meta
Case history gastric cancer
Metastastatic ability Yes
Classification cancer
Year of origin 1991
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 59-65(50) : 59(3),60(4),61(8),62(20),63(11),64(3)/
deposit info
lot info
Reference information Reference 1
User's Publication 4

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2691  Uesugi H, Yamashita K, Atari E, Shinoda H, Nishiyama T, Okayasu I, Yasuda S  [Establishment and characterization of strain KE-39 derived from human gastric cancer]  Hum Cell  1994  7:227-32  PubMed ID: 7599112

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User's Publication
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217
5837  Liu J et al  Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.  Nat Commun  2014  5:3830  PubMed ID: 24807215
5845  Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.  Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.  PLoS One  2013  8(2):e56766  PubMed ID: 23451082
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309

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