Back Back
Cell No. : Cell Name
RCB1426 : PMF-ko14  update : 2024/05/08
CommentStopped distribution.. Until September 2023, we have distributed this cell line as “Japanese colon carcinoma cell line. Family of the patient also exhibited high risk, but not so-called HNPCC.” This cell line was a mix-up of KM12, a colon carcinoma cell line registered in the National Cancer Institute of USA.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Okazaki, Keisuke
Originator Okazaki, Keisuke
Year of deposit 1998
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 51 years
Tissue colon
Metastatic ability Yes
Metastatic tissue peritoneum, spleen
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8747
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 78-84(50) : /79(5), 80(6), 81(12), 82(10). 83(11)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 6

To topTop
2454  Okazaki K, Nakayama Y, Shibao K, Hirata K, Sako T, Nagata N, Kuroda Y, Itoh H  Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.  Int J Oncol  2000  17:39-45  PubMed ID: 10853016   DOI: 10.3892/ijo.17.1.39

To topTop
User's Publication
20792  Nittayaboon K, Leetanaporn K, Sangkhathat S, Roytrakul S, Navakanitworakul R.  Cytotoxic effect of metformin on butyrate-resistant PMF-K014 colorectal cancer spheroid cells.  Biomed Pharmacother  2022  151:113214  PubMed ID: 35676792   DOI: 10.1016/j.biopha.2022.113214
10027  Yasuyuki Okada, Tetsuo Kimura, Tadahiko Nakagawa, Koichi Okamoto, Akira Fukuya, Takahiro Goji, Shota Fujimoto, Masahiro Sogabe, Hiroshi Miyamoto, Naoki Muguruma, Yasushi Tsuji, Toshiya Okahisa, Tetsuji Takayama  EGFR Downregulation After Anti-EGFR Therapy Predicts the Antitumor Effect in Colorectal Cancer  Mol Cancer Res  2017  15(10):1445-1454  PubMed ID: 28698359   DOI: 10.1158/1541-7786.MCR-16-0383
14588  Suzuki H, Sasada M, Kamiya S, Ito Y, Watanabe H, Okada Y, Ishibashi K, Iyoda T, Yanaka A, Fukai F.  The Promoting Effect of the Extracellular Matrix Peptide TNIIIA2 Derived from Tenascin-C in Colon Cancer Cell Infiltration  Int J Mol Sci  2017  18(1):181  PubMed ID: 28106752   DOI: 10.3390/ijms18010181
18293  Ohta M, Seto M, Ijichi H, Miyabayashi K, Kudo Y, Mohri D, Asaoka Y, Tada M, Tanaka Y, Ikenoue T, Kanai F, Kawabe T, Omata M.  Decreased expression of the RAS-GTPase activating protein RASAL1 is associated with colorectal tumor progression  Gastroenterology  2009  136(1):206-16  PubMed ID: 18992247   DOI: 10.1053/j.gastro.2008.09.063
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
4723  Minagawa N, Nakayama Y, Inoue Y, Onitsuka K, Katsuki T, Tsurudome Y, Shibao K, Hirata K, Sako T, Nagata N, Ohie S, Kohno K, Itoh H.  4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid inhibits angiogenesis in colon cancer through reduced expression of vascular endothelial growth factor.  Oncol Res  2004  14(9):407-14  PubMed ID: 15490972   DOI: 10.3727/0965040041791464

Back Back Return Top Page