Cell No. : Cell Name
RCB1426 : PMF-ko14
update : 2023/11/02
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| Comment | Stopped distribution.. Until September 2023, we have distributed this cell line as “Japanese colon carcinoma cell line. Family of the patient also exhibited high risk, but not so-called HNPCC.” This cell line was a mix-up of KM12, a colon carcinoma cell line registered in the National Cancer Institute of USA. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Okazaki, Keisuke
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| Originator |
Okazaki, Keisuke
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| Year of deposit |
1998
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Female
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| Age at sampling |
51 years
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| Tissue |
colon
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| Metastatic ability |
Yes
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| Metastatic tissue |
peritoneum, spleen
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| Classification |
cancer
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| Year of origin |
1996
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_8747
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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78-84(50) : /79(5), 80(6), 81(12), 82(10). 83(11)/
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
6
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| User's Publication |
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20792
Nittayaboon K, Leetanaporn K, Sangkhathat S, Roytrakul S, Navakanitworakul R.
Cytotoxic effect of metformin on butyrate-resistant PMF-K014 colorectal cancer spheroid cells.
Biomed Pharmacother
2022
151:113214
PubMed ID: 35676792
DOI: 10.1016/j.biopha.2022.113214
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10027
Yasuyuki Okada, Tetsuo Kimura, Tadahiko Nakagawa, Koichi Okamoto, Akira Fukuya, Takahiro Goji, Shota Fujimoto, Masahiro Sogabe, Hiroshi Miyamoto, Naoki Muguruma, Yasushi Tsuji, Toshiya Okahisa, Tetsuji Takayama
EGFR Downregulation After Anti-EGFR Therapy Predicts the Antitumor Effect in Colorectal Cancer
Mol Cancer Res
2017
15(10):1445-1454
PubMed ID: 28698359
DOI: 10.1158/1541-7786.MCR-16-0383
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14588
Suzuki H, Sasada M, Kamiya S, Ito Y, Watanabe H, Okada Y, Ishibashi K, Iyoda T, Yanaka A, Fukai F.
The Promoting Effect of the Extracellular Matrix Peptide TNIIIA2 Derived from Tenascin-C in Colon Cancer Cell Infiltration
Int J Mol Sci
2017
18(1):181
PubMed ID: 28106752
DOI: 10.3390/ijms18010181
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18293
Ohta M, Seto M, Ijichi H, Miyabayashi K, Kudo Y, Mohri D, Asaoka Y, Tada M, Tanaka Y, Ikenoue T, Kanai F, Kawabe T, Omata M.
Decreased expression of the RAS-GTPase activating protein RASAL1 is associated with colorectal tumor progression
Gastroenterology
2009
136(1):206-16
PubMed ID: 18992247
DOI: 10.1053/j.gastro.2008.09.063
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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4723
Minagawa N, Nakayama Y, Inoue Y, Onitsuka K, Katsuki T, Tsurudome Y, Shibao K, Hirata K, Sako T, Nagata N, Ohie S, Kohno K, Itoh H.
4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid inhibits angiogenesis in colon cancer through reduced expression of vascular endothelial growth factor.
Oncol Res
2004
14(9):407-14
PubMed ID: 15490972
DOI: 10.3727/0965040041791464
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