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Cell No. : Cell Name
RCB1426 : PMF-ko14  update : 2021/01/08
CommentJapanese colon carcinoma. Family of the patient also exhibited high risk, but not so-called HNPCC.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Okazaki, Keisuke
Originator Okazaki, Keisuke
Year of deposit 1998
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 51 years
Tissue colon
Metastastatic ability Yes
Metastatsd tissue peritoneum, spleen
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method (0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 78-84(50) : /79(5), 80(6), 81(12), 82(10). 83(11)/
deposit info
lot info
Reference information Reference 1
User's Publication 3

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2454  Okazaki K, Nakayama Y, Shibao K, Hirata K, Sako T, Nagata N, Kuroda Y, Itoh H  Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.  Int J Oncol  2000  17:39-45  PubMed ID: 10853016

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User's Publication
10027  Yasuyuki Okada, Tetsuo Kimura, Tadahiko Nakagawa, Koichi Okamoto, Akira Fukuya, Takahiro Goji, Shota Fujimoto, Masahiro Sogabe, Hiroshi Miyamoto, Naoki Muguruma, Yasushi Tsuji, Toshiya Okahisa, Tetsuji Takayama  EGFR Downregulation After Anti-EGFR Therapy Predicts the Antitumor Effect in Colorectal Cancer  Mol Cancer Res  2017  15(10):1445-1454  PubMed ID: 28698359
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
4723  Minagawa N, Nakayama Y, Inoue Y, Onitsuka K, Katsuki T, Tsurudome Y, Shibao K, Hirata K, Sako T, Nagata N, Ohie S, Kohno K, Itoh H.  4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid inhibits angiogenesis in colon cancer through reduced expression of vascular endothelial growth factor.  Oncol Res  2004  14(9):407-14  PubMed ID: 15490972

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