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Cell No. : Cell Name
RCB1425 : LCAM1  update : 2022/08/24
CommentHuman lung cancer derived cell.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Masayoshi
Originator Inoue, Masayoshi
Year of deposit 1998
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 53 years
Tissue lung
Disease name lung cancer
Classification cancer
Year of origin 1995
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E062
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 53-61(50) : /56(8),57(8),58(13),59(9),60(6)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
2675  Inoue M, Minami M, Fujii Y, Matsuda H, Shirakura R, Kido T  Granulocyte colony-stimulating factor and interleukin-6-producing lung cancer cell line, LCAM.  J Surg Oncol  1997  64:347-50  PubMed ID: 9142195   DOI: 10.1002/(sici)1096-9098(199704)64:4<347::aid-jso18>3.0.co;2-#

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User's Publication
10922  Shigeaki Umeda et al  Loss of IL-24 expression facilitates invasion in lung adenocarcinoma   Int J Clin Exp Pathol  2017  10:173-185   
16553  Hayashi S, Kumai T, Matsuda Y, Aoki N, Sato K, Kimura S, Kitada M, Tateno M, Celis E, Kobayashi H.  Six-transmembrane epithelial antigen of the prostate and enhancer of zeste homolog 2 as immunotherapeutic targets for lung cancer  J Transl Med  2011  9:191  PubMed ID: 22053850   DOI: 10.1186/1479-5876-9-191



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