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Cell No. : Cell Name
RCB1423 : BHK-21  update : 2022/04/01
CommentThe parent cell line used for the temperature sensitive cell line series of BN.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishimoto, Takeharu
Year of deposit 1998
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification other
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_1915
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 15

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18  Macpherson,I. and Stoker,M.  Polyoma transformation of hamster cell clones----an investigation of genetic factors affecting cell competence.  Virology  1962  16:147-151  PubMed ID: 14468055   DOI: 10.1016/0042-6822(62)90290-8

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User's Publication
19956  Mixich L, Boonstra E, Masuda K, Li SW, Nakashima Y, Meng F, Sakata M, Goda T, Uchida S, Cabral H.  Ionizable Polymeric Micelles with Phenylalanine Moieties Enhance Intracellular Delivery of Self-Replicating RNA for Long-Lasting Protein Expression In Vivo  Biomacromolecules  2024  Jan 5  PubMed ID: 38181450   DOI: 10.1021/acs.biomac.3c01102
20942  Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B.  A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.  J Cell Biol  2023  222(2):e202207147  PubMed ID: 36394541   DOI: 10.1083/jcb.202207147
21359  Nakamura M, Araki H, Tsurushima H, Oyane A.  Fabrication of water-dispersible and cell-stimulating calcium phosphate nanoparticles immobilizing basic fibroblast growth factor.  Colloids Surf B Biointerfaces  2023  230:113502  PubMed ID: 37574618   DOI: 10.1016/j.colsurfb.2023.113502
20727  Nakajima KP, Valansi C, Kurihara D, Sasaki N, Podbilewicz B, Higashiyama T.  Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules.  Sci Rep  2022  12(1):9609  PubMed ID: 35688940   DOI: 10.1038/s41598-022-13547-w
6934  Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T.  Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.  Histochem Cell Biol  2020    PubMed ID: 31907597   DOI: 10.1007/s00418-019-01842-z
10648  Tezuka K, Okuma K, Kuramitsu M, Matsuoka S, Tanaka R, Tanaka Y, Hamaguchi I.  Control of Human T-Cell Leukemia Virus Type 1 (HTLV-1) Infection by Eliminating Envelope Protein-Positive Cells with Recombinant Vesicular Stomatitis Viruses Encoding HTLV-1 Primary Receptor.  J. Virol.  2018  92:  PubMed ID: 29212930   DOI: 10.1128/JVI.01885-17
10041  Okuma K, Fukagawa K, Kohma T, Takahama Y, Hamaguchi Y, Ito M, Tanaka Y, Buonocore L, Rose JK, Hamaguchi I.  A recombinant vesicular stomatitis virus encoding CCR5-tropic HIV-1 receptors targets HIV-1-infected cells and controls HIV-1 infection.  Microbes Infect.  2017  19:277-287  PubMed ID: 28025070   DOI: 10.1016/j.micinf.2016.12.004
7304  Matsuo E, Saeki K, Roy P, Kawano J.  Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV.  FEBS Open Bio  2015  5:445-53  PubMed ID: 26101741   DOI: 10.1016/j.fob.2015.05.006
7871  Imai S, Sudo Y, Nakamura A, Ozeki A, Asato M, Hojo M, Devi LA, Kuzumaki N, Suzuki T, Uezono Y, Narita M.  Possible involvement of β-endorphin in a loss of the coordinated balance of μ-opioid receptors trafficking processes by fentanyl.  Synapse  2011  65:962-6  PubMed ID: 21437993   DOI: 10.1002/syn.20930
14409  Tsuruno C, Okuma K, Takahashi Y, Tanaka R, Tanaka Y, Takahama Y, Hamaguchi Y, Hamaguchi I, Yamaguchi K.  A recombinant vesicular stomatitis virus encoding HIV-1 receptors and human OX40 ligand efficiently eliminates HIV-1-infected CD4-positive T cells expressing OX40  Hum Immunol   2011  72(4):295-304  PubMed ID: 21262309   DOI: 10.1016/j.humimm.2011.01.007
15351  Terao Y, Ayaori M, Ogura M, Yakushiji E, Uto-Kondo H, Hisada T, Ozasa H, Takiguchi S, Nakaya K, Sasaki M, Komatsu T, Iizuka M, Horii S, Mochizuki S, Yoshimura M, Ikewaki K.  Effect of sulfonylurea agents on reverse cholesterol transport in vitro and vivo  J Atheroscler Thromb  2011  18(6):513-30  PubMed ID: 21636950   DOI: 10.5551/jat.7641
12808  Oyane A, Tsurushima H, Ito A.  Novel gene-transferring scaffolds having a cell adhesion molecule-DNA-apatite nanocomposite surface  Gene Ther  2007  14(24):1750-3  PubMed ID: 17943146   DOI: 10.1038/
3383  Yamanaka, Ryuya, Xanthopoulos, Kleanthis G  Development of improved Sindbis virus-based DNA expression vector.  DNA Cell Biol  2004  23:75-80  PubMed ID: 15000747   DOI: 10.1089/104454904322759885
18762  Yahiro K, Wada A, Yamasaki E, Nakayama M, Nishi Y, Hisatsune J, Morinaga N, Sap J, Noda M, Moss J, Hirayama T.  Essential domain of receptor tyrosine phosphatase beta (RPTPbeta) for interaction with Helicobacter pylori vacuolating cytotoxin  J Biol Chem  2004  279(49):51013-21  PubMed ID: 15383529   DOI: 10.1074/jbc.M406473200
9239  Ogawa A, Tomita N, Kikuchi N, Sando S, Aoyama Y.  Aptamer selection for the inhibition of cell adhesion with fibronectin as target.  Bioorg. Med. Chem. Lett.  2004  14:4001-4  PubMed ID: 15225715   DOI: 10.1016/j.bmcl.2004.05.042

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