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Cell No. : Cell Name
RCB1422 : L929  update : 2023/06/30
CommentTNFalpha-sensitive L929. Do not confuse with RCB0081 L929 that is insensitive to TNFalpha.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Year of deposit 1997
Another name NCTC Clone 929 (JCRB9003)
Animal _mouse < Mammals
Genus Mus
Species musculus
Tissue connective tissue
Classification other
History JCRB--->RIKEN
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0462
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% HS (GIBCO) + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 16

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537  Stanley ER, Heard PM.  Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.  J Biol Chem  1977  252(12):4305-12  PubMed ID: 301140  
4661  WAYMOUTH C.  Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).  J Natl Cancer Inst  1959  22(5):1003-17  PubMed ID: 13655076   DOI: 10.1093/jnci/22.5.1003
1817  SANFORD KK, HOBBS GL, EARLE WR.  The tumor-producing capacity of strain L mouse cells after 10 years in vitro.  Cancer Res  1956  16(2):162-6  PubMed ID: 13293658  
4660  EAGLE H.  The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.  J Exp Med  1955  102(5):595-600  PubMed ID: 13271674   DOI: 10.1084/jem.102.5.595
1818  EARLE WR, SCHILLING EL, BRYANT JC, EVANS VJ.  The growth of pure strain L cells in fluid-suspension cultures.  J Natl Cancer Inst.  1954  14(5):1159-71  PubMed ID: 13233852  
4659  EVANS VJ, SHANNON JE Jr, BRYANT JC, WALTZ HK, EARLE WR, SANFORD KK.  A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.  J Natl Cancer Inst  1953  13(4):773-84  PubMed ID: 13035407  
4658  SANFORD KK, EARLE WR, LIKELY GD.  The growth in vitro of single isolated tissue cells.  J Natl Cancer Inst  1948  9(3):229-46  PubMed ID: 18105872  
4657  Earle, W.R  Production of malignancy in vitro.Ⅳ.The mouse fibroblast cultures and changes seen in the living cells.  J. Nat. Cancer Inst.  1943  4:165-212   

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User's Publication
21720  Baba H, Fujita T, Mizuno K, Tambo M, Toda S.  Programming Spatial Cell Sorting by Engineering Cadherin Intracellular Activity.  ACS Synth Biol  2024    PubMed ID: 38726686   DOI: 10.1021/acssynbio.3c00774
21554  Akiyama Y, Harada K, Miyakawa J, Kreder KJ, O'Donnell MA, Daichi M, Katoh H, Hori M, Owari K, Futami K, Ishikawa S, Ushiku T, Kume H, Homma Y, Luo Y.  Th1/17 polarization and potential treatment by an anti-interferon-γ DNA aptamer in Hunner-type interstitial cystitis.  iScience  2023  26(11):108262  PubMed ID: 38026177   DOI: 10.1016/j.isci.2023.108262
16508  Toyama C, Maeda A, Kogata S, Takase K, Kodama T, Masahata K, Ueno T, Kamiyama M, Tazuke Y, Eguchi H, Matsunami K, Miyagawa S, Okuyama H.  Effect of a C5a receptor antagonist on macrophage function in an intestinal transplant rat model  Transpl Immunol  2022  101559  PubMed ID: 35227893   DOI: 10.1016/j.trim.2022.101559
20767  Yoshikawa C, Takagi R, Nakaji-Hirabayashi T, Ochi T, Kawamura Y, Thissen H.  Marine Antifouling Coatings Based on Durable Bottlebrush Polymers.  ACS Appl Mater Interfaces  2022    PubMed ID: 35816694   DOI: 10.1021/acsami.2c06647
20026  Kido Y, Motomura H, Ikeda Y, Satoh S, Jinno M.  Clarification of electrical current importance in plasma gene transfection by equivalent circuit analysis.  PLoS One  2021  16(1):e0245654  PubMed ID: 33508006   DOI: 10.1371/journal.pone.0245654
9898  Tasuku Kodama, Akira Maeda, Pei-Chi Lo, Yuki Noguchi, Chiyoshi Toyama, Yuichi Takama, Takehisa Ueno, Yuko Tazuke, Hiroshi Eguchi, Katsuyoshi Matsunami, Shuji Miyagawa, Hiroomi Okuyama  The Effect of a Novel Immunosuppressive Drug, a PAK-2 Inhibitor, on Macrophage Differentiation/Polarization in a Rat Small Intestinal Transplantation Model  Transpl Immunol  2019  57:101246  PubMed ID: 31526866   DOI: 10.1016/j.trim.2019.101246
10672  Mukai H, Takahashi M, Watanabe Y.  Potential usefulness of Brevibacillus for bacterial cancer therapy: intratumoral provision of tumor necrosis factor-α and anticancer effects.  Cancer Gene Ther.  2018  25(3-4):47-57  PubMed ID: 29255215   DOI: 10.1038/s41417-017-0009-7
10967    Synergistic effect of electrical and chemical factors on endocytosis in micro-discharge plasma gene transfection  Plasma Sources Science and Technology  2017  26    DOI: 10.1088/1361-6595/aa70de
14194  Naoki Tanigawa, Sachiko Yano, Akira Higashibata, Yoshikazu Tsuchiya, Haruna Tanii, Noboru Ando, Koji Kubota, Mutsumu Nagase  Development and Validation of a Closed Chamber for Cell Culture Experiments in Space  Biological Sciences in Space  2014  28:1-5    DOI: 10.2187/bss.28.1
7840  Kurita K, Matsumura Y, Takahara H, Hatta K, Shimojoh M.  Synthesis and macrophage activation of lentinan-mimic branched amino polysaccharides: curdlans having N-Acetyl-d-glucosamine branches.  Biomacromolecules  2011  12:2267-74  PubMed ID: 21526779   DOI: 10.1021/bm200353m
8828  Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363   DOI: 10.1099/vir.0.82535-0
8842  Takahiro Hirano, Taichi Murakami, Masayuki Taira, Takayuki Narushima and Chiaki Ouchi  Alloy Design and Properties of New α+β Titanium Alloy with Excellent Cold Workability, Superplasticity and Cytocompatibility  ISIJ International  2007  47:745-752    DOI: 10.2355/isijinternational.47.745
6286  Kaori Taneichi, Masayuki Taira, Eichi Sukedai, Takayuki Narushima, Yasutaka Iguchi, Chiaki Ouchi  Alloy Design and Property Evaluation of New β Type Titanium Alloy with Excellent Cold Workability and Biocompatibility  ISIJ International  2006  46:292-301    DOI: 10.2355/isijinternational.46.292
10075  Masaji Kawatsu, Jutaro Wada, Mitsuaki Kitano, Eiji Ogino, Hiroshi Sakurai, Shigeo Furuyoshi, Nobutaka Tani  Effects of a New Extracorporeal System Using CTR on Mortality and Inflammatory Responses to Bacterial Toxin-Induced Multiple Organ Dysfunction Syndrome in Rabbits  Blood Purif  2006  24(3):327-34  PubMed ID: 16534195   DOI: 10.1159/000091997
2547  Shoji Y, Inoue Y, Sugisawa H, Itou T, Endo T, Sakai T.  Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha.  Vet Immunol Immunopathol  2001  82(3-4):183-92  PubMed ID: 11587733   DOI: 10.1016/s0165-2427(01)00353-1
16946  Takekoshi M, Maeda F, Nagatsuka Y, Aotsuka S, Ono Y, Ihara S.  Cloning and expression of human anti-tumor necrosis factor-alpha monoclonal antibodies from Epstein-Barr virus transformed oligoclonal libraries  J Biochem   2001  130(2):299-303  PubMed ID: 11481049   DOI: 10.1093/oxfordjournals.jbchem.a002986

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