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Cell No. : Cell Name
RCB1378 : MuSS  update : 2022/05/30
CommentA-Jackson mouse-derived histiocyte-like cells from spontaneous sarcoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Watanabe, Itaru
Originator Watanabe, Itaru
Year of deposit 1997
Another name Murine Sarcoma Sendai
Animal _mouse < Mammals
Strain name A/J
Gender Male
Age at sampling 60 weeks
Tissue muscle
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8736
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2210  Watanabe I, Kurosawa N, Nishihira T.  Establishment and characterization of a murine cell-line derived from malignant fibrous histiocytoma of A/Jackson mouse.  Tohoku J Exp Med  1998  184(3):173-87  PubMed ID: 9591335   DOI: 10.1620/tjem.184.173

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User's Publication
14536  Kawakubo N, Tanaka S, Kinoshita Y, Tajiri T, Yonemitsu Y, Taguchi T.  Sequential actions of immune effector cells induced by viral activation of dendritic cells to eliminate murine neuroblastoma  J Pediatr Surg  2018  53(8):1615-1620  PubMed ID: 28941928   DOI: 10.1016/j.jpedsurg.2017.08.019



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