Cell No. : Cell Name
RCB1377 : 32Dcl3
update : 2022/04/04
|
| Comment | Mouse IL-3 dependent cells cloned from 32D. Differentiate with G-CSF stimulation. Conditioned medium. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Todokoro, Kazuo
|
| Year of deposit |
1997
|
| Original cell |
32D
|
| Animal |
mouse
< Mammals
|
| Strain name |
C3H/HeJ
|
| Tissue |
bone marrow
|
| Disease name |
myeloid precursor cell
|
| Classification |
transformed
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_0119
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Medium and additives |
|
RPMI1640 + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
9
|
| User's Publication |
1
|
| Reference |
|
5470
Patel G, Kreider B, Rovera G, Reddy EP.
v-myb blocks granulocyte colony-stimulating factor-induced myeloid cell differentiation but not proliferation.
Mol Cell Biol
1993
13(4):2269-76
PubMed ID: 7681145
|
|
2259
Kreider BL, Benezra R, Rovera G, Kadesch T.
Inhibition of myeloid differentiation by the helix-loop-helix protein Id.
Science
1992
255(5052):1700-2
PubMed ID: 1372755
|
|
5468
Friedman AD, Krieder BL, Venturelli D, Rovera G.
Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.
Blood
1991
78(9):2426-32
PubMed ID: 1718497
|
|
2260
Kreider BL, Phillips PD, Prystowsky MB, Shirsat N, Pierce JH, Tushinski R, Rovera G.
Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.
Mol Cell Biol
1990
10(9):4846-53
PubMed ID: 1697033
|
|
5466
Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.
Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.
J Cell Biol
1989
109(2):833-41
PubMed ID: 2668305
|
|
4924
Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.
Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.
Ann N Y Acad Sci
1987
511:30-8
PubMed ID: 2830826
|
|
4925
Rovera G, Valtieri M, Mavilio F, Reddy EP.
Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.
Oncogene
1987
1(1):29-35
PubMed ID: 2449644
|
|
2770
Valtieri M, Tweardy DJ, Caracciolo D, Johnson K, Mavilio F, Altmann S, Santoli D, Rovera G.
Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.
J Immunol
1987
138(11):3829-35
PubMed ID: 2438328
|
|
1979
Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J
Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.
Proc Natl Acad Sci U S A
1983
80:2931-5
PubMed ID: 6574462
|
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