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Cell No. : Cell Name
RCB1377 : 32Dcl3  update : 2024/01/17
CommentMouse IL-3 dependent cells cloned from 32D. Differentiate with G-CSF stimulation. Conditioned medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Todokoro, Kazuo
Year of deposit 1997
Original cell 32D
Animal _mouse < Mammals
Strain name C3H/HeJ
Tissue bone marrow
Disease name myeloid precursor cell
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0119
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium)
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 9
User's Publication 3

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5470  Patel G, Kreider B, Rovera G, Reddy EP.  v-myb blocks granulocyte colony-stimulating factor-induced myeloid cell differentiation but not proliferation.  Mol Cell Biol  1993  13(4):2269-76  PubMed ID: 7681145   DOI: 10.1128/mcb.13.4.2269
2259  Kreider BL, Benezra R, Rovera G, Kadesch T.  Inhibition of myeloid differentiation by the helix-loop-helix protein Id.  Science  1992  255(5052):1700-2  PubMed ID: 1372755   DOI: 10.1126/science.1372755
5468  Friedman AD, Krieder BL, Venturelli D, Rovera G.  Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.  Blood  1991  78(9):2426-32  PubMed ID: 1718497  
2260  Kreider BL, Phillips PD, Prystowsky MB, Shirsat N, Pierce JH, Tushinski R, Rovera G.  Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.  Mol Cell Biol  1990  10(9):4846-53  PubMed ID: 1697033   DOI: 10.1128/mcb.10.9.4846
5466  Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.  Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.  J Cell Biol  1989  109(2):833-41  PubMed ID: 2668305   DOI: 10.1083/jcb.109.2.833
4924  Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.  Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.  Ann N Y Acad Sci  1987  511:30-8  PubMed ID: 2830826   DOI: 10.1111/j.1749-6632.1987.tb36235.x
4925  Rovera G, Valtieri M, Mavilio F, Reddy EP.  Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.  Oncogene  1987  1(1):29-35  PubMed ID: 2449644  
2770  Valtieri M, Tweardy DJ, Caracciolo D, Johnson K, Mavilio F, Altmann S, Santoli D, Rovera G.  Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.  J Immunol  1987  138(11):3829-35  PubMed ID: 2438328  
1979  Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J  Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.  Proc Natl Acad Sci U S A  1983  80:2931-5  PubMed ID: 6574462   DOI: 10.1073/pnas.80.10.2931

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User's Publication
21407  Umene K, Nagamune T, Kawahara M.  Phenotypic screening of signaling motifs that efficiently induce cell proliferation.  Sci Rep  2023  13(1):15639  PubMed ID: 37730760   DOI: 10.1038/s41598-023-42378-6
20985  Nakajima K, Shen Z, Miura M, Nakabayashi H, Kawahara M.  Sequential control of myeloid cell proliferation and differentiation by cytokine receptor-based chimeric antigen receptors.  PLoS One  2022  17(12):e0279409  PubMed ID: 36574389   DOI: 10.1371/journal.pone.0279409
13975  Chen F, Ishikawa Y, Akashi A, Naoe T, Kiyoi H.  Co-expression of wild-type FLT3 attenuates the inhibitory effect of FLT3 inhibitor on FLT3 mutated leukemia cells  Oncotarget  2016  7(30):47018-47032  PubMed ID: 27331411   DOI: 10.18632/oncotarget.10147

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