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Cell No. : Cell Name
RCB1376 : 2-7  update : 2022/05/30
CommentA copy of CAG-loxP-neopA-loxP-LacZ was inserted. Cre will delete neopA and induce LacZ. Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Saito, Izumi
Originator Kanegae, Yumi
Year of deposit 1997
Animal monkey < Mammals
Strain name African green
Genus Cercopithecus
Species aethiops
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene pCAGGS
History originator -> Saito, Izumi
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T366
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS + 1mg/ml G418
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
2217  Kanegae Y, Takamori K, Sato Y, Lee G, Nakai M, Saito I  Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase.  Gene  1996  181:207-12  PubMed ID: 8973332  

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