Back
|
Cell No. : Cell Name
RCB1376 : 2-7 update : 2024/11/14 |
||||
| Comment | CITES. A copy of CAG-loxP-neopA-loxP-LacZ was inserted. Cre will delete neopA and induce LacZ. Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES). Origin animal species details | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Saito, Izumi | ||
| Originator | Kanegae, Yumi | |||
| Year of deposit | 1997 | |||
| Animal | _monkey < Mammals | |||
| Strain name | African green Origin animal species details | |||
| Genus | Cercopithecus | |||
| Species | aethiops | |||
| Tissue | kidney | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | pCAGGS | |||
| History | originator -> Saito, Izumi | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_T366 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM (low glucose) + 10% FBS + 1mg/ml G418 | |||
| Antibiotics | Free | Passage method | (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA) | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
Top
Top
Back
CELL BANK Website