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Cell No. : Cell Name
RCB1374 : 1-8  update : 2024/11/14
CommentCITES. CAG-loxP-neopA-loxP-LacZ(incomplete) was inserted. Control cell line for 2-2 and 2-7 cells. Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES). Origin animal species details
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Saito, Izumu
Originator Kanegae, Yumi
Year of deposit 1997
Animal _monkey < Mammals
Strain name African green Origin animal species details
Genus Cercopithecus
Species aethiops
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene pCAGGS
History originator -> Saito, Izumi
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T367
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS + 1mg/ml G418
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
2217  Kanegae Y, Takamori K, Sato Y, Lee G, Nakai M, Saito I  Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase.  Gene  1996  181:207-12  PubMed ID: 8973332   DOI: 10.1016/s0378-1119(96)00516-1

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