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Cell No. : Cell Name
RCB1373 : NBT-L2b  update : 2022/04/01
CommentA subline of NBT-II. The most invasive bladder carcinoma. Relatively low cadherin expression.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Nozomu
Year of deposit 1997
Animal _rat < Mammals
Strain name Wistar
Genus Rattus
Species norvegicus
Gender Male
Tissue bladder
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4673
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, MPI, PepB
deposit info
lot info
Reference information Reference 2
User's Publication 3

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2491  Nishi N, Inui M, Kishi Y, Miyanaka H, Wada F  Isolation and Characterization of Invasive and Noninvasive of a Rat Bladder Tumor Cell Line  Jpn J Cancer Res  1997  88:831-838  PubMed ID: 9369930   DOI: 10.1111/j.1349-7006.1997.tb00458.x
2492  Toyoshima K, Ito N, Hiasa Y, Kamamoto Y, Makiura S  Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(-4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II.  J Natl Cancer Inst  1971  47:979-85  PubMed ID: 4941696  

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User's Publication
7496  Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Miyake M, Miyake J, Fujita S.  Identification of kinases and regulatory proteins required for cell migration using a transfected cell-microarray system.  BMC Genet.  2015  16:9  PubMed ID: 25652422   DOI: 10.1186/s12863-015-0170-7
15971  Satoshi Fujita, Reiko Onuki-Nagasaki, Junji Fukuda, Junko Enomoto, Suichi Yamaguchi, Masato Miyake  Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing  Lab Chip  2013  13:77-80  PubMed ID: 23165644   DOI: 10.1039/c2lc40709d
8303  Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J.  Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.  Methods Mol. Biol.  2010  629:193-203  PubMed ID: 20387151   DOI: 10.1007/978-1-60761-657-3_13

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