Cell No. : Cell Name RCB1373 : NBT-L2b  update : 2022/04/01
Comment			A subline of NBT-II. The most invasive bladder carcinoma. Relatively low cadherin expression.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Nishi, Nozomu
		Year of deposit	1997
		Animal	_rat < Mammals
		Strain name	Wistar
		Genus	Rattus
		Species	norvegicus
		Gender	Male
		Tissue	bladder
		Classification	cancer
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_4673
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 1-2 times/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, AST, MPI, PepB
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	3

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Reference

2491  Nishi N, Inui M, Kishi Y, Miyanaka H, Wada F  Isolation and Characterization of Invasive and Noninvasive of a Rat Bladder Tumor Cell Line  Jpn J Cancer Res  1997  88:831-838  PubMed ID: 9369930   DOI: 10.1111/j.1349-7006.1997.tb00458.x

2492  Toyoshima K, Ito N, Hiasa Y, Kamamoto Y, Makiura S  Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(-4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II.  J Natl Cancer Inst  1971  47:979-85  PubMed ID: 4941696

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User's Publication

7496  Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Miyake M, Miyake J, Fujita S.  Identification of kinases and regulatory proteins required for cell migration using a transfected cell-microarray system.  BMC Genet.  2015  16:9  PubMed ID: 25652422   DOI: 10.1186/s12863-015-0170-7

15971  Satoshi Fujita, Reiko Onuki-Nagasaki, Junji Fukuda, Junko Enomoto, Suichi Yamaguchi, Masato Miyake  Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing  Lab Chip  2013  13:77-80  PubMed ID: 23165644   DOI: 10.1039/c2lc40709d

8303  Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J.  Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.  Methods Mol. Biol.  2010  629:193-203  PubMed ID: 20387151   DOI: 10.1007/978-1-60761-657-3_13