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Cell No. : Cell Name
RCB1372 : NBT-L2a  update : 2022/04/01
CommentA subline of NBT-II. Highly invasive bladder carcinoma. Relatively low cadherin expression.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Nozomu
Originator NIshi, Nozomu
Year of deposit 1997
Animal rat < Mammals
Strain name Wistar
Genus Rattus
Species norvegicus
Tissue bladder
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4672
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, MPI, PepB
Reference information Reference 2
User's Publication 0


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Reference
2491  Nishi N, Inui M, Kishi Y, Miyanaka H, Wada F  Isolation and Characterization of Invasive and Noninvasive of a Rat Bladder Tumor Cell Line  Jpn J Cancer Res  1997  88:831-838  PubMed ID: 9369930  
2492  Toyoshima K, Ito N, Hiasa Y, Kamamoto Y, Makiura S  Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(-4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II.  J Natl Cancer Inst  1971  47:979-85  PubMed ID: 4941696  

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