Cell No. : Cell Name RCB1370 : NBT-T2  update : 2026/06/19
Comment			Stopped distribution temporarily.A subline of NBT-II. Almost non-invasive bladder carcinoma. Relatively high cadherin expression.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). Regarding commercial use or use related to patent applications, please first contact the RIKEN BRC at cellbank.brc@riken.jp.
	Basic information	Depositor	Nishi, Nozomu
		Originator	Nishi, Nozomu
		Year of deposit	1997
		Animal	_rat < Mammals
		Strain name	Wistar
		Genus	Rattus
		Species	norvegicus
		Gender	Male
		Tissue	bladder
		Classification	cancer
		Lifespan	infinite
		Morphology	epithelial-like
		SDS	SDS_DMSO(Japanese)
		Cellosaurus(Expasy)	CVCL_4675
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, AST, MPI, PepB
Reference information		Reference	2
		User's Publication	9

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Reference

2491  Nishi N, Inui M, Kishi Y, Miyanaka H, Wada F  Isolation and Characterization of Invasive and Noninvasive of a Rat Bladder Tumor Cell Line  Jpn J Cancer Res  1997  88:831-838  PubMed ID: 9369930   DOI: 10.1111/j.1349-7006.1997.tb00458.x

2492  Toyoshima K, Ito N, Hiasa Y, Kamamoto Y, Makiura S  Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(-4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II.  J Natl Cancer Inst  1971  47:979-85  PubMed ID: 4941696

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User's Publication

22610  Kobayashi S, Kashiwagi H, Kobayashi K, Kohzaki M.  4,4'-methylenebis(2-chloroaniline) induces chromosome aneuploidy associated with premature chromatid separation in mammalian cells: A possible carcinogenic mechanism.  Ecotoxicol Environ Saf  2025  293:117981  PubMed ID: 40073788   DOI: 10.1016/j.ecoenv.2025.117981

13594  Hanif N, Murni A, Tanaka J.  Sangiangols A and B, Two New Dolabellanes from an Indonesian Marine Soft Coral, Anthelia sp  Molecules  2020  25(17):3803  PubMed ID: 32825594   DOI: 10.3390/molecules25173803

19455  Trianto A, Hermawan I, Suzuka T, Tanaka J.  Two new cytotoxic candidaspongiolides from an indonesian sponge  ISRN Pharm  2011  2011:852619  PubMed ID: 22389864   DOI: 10.5402/2011/852619

19456  Uddin MH, Hanif N, Trianto A, Agarie Y, Higa T, Tanaka J.  Four new polyoxygenated gorgosterols from the gorgonian Isis hippuris  Nat Prod Res  2011  25(6):585-91  PubMed ID: 21409719   DOI: 10.1080/14786419.2010.485303

19457  Hermawan I, de Voogd NJ, Tanaka J.  An acetylenic alkaloid from the calcareous sponge Leucetta sp  Mar Drugs  2011  9(3):382-6  PubMed ID: 21556167   DOI: 10.3390/md9030382

19458  Mohammad Helal Uddin, Michael Chandro Roy, Junichi Tanaka  Cytotoxic cholic acid type sterones from a marine soft coral Paraminabea sp.  Chemistry of Natural Compounds  2011  47:64-67    DOI: 10.1007/s10600-011-9831-6

16166  Trianto A, Hermawan I, de Voogd NJ, Tanaka J.  Halioxepine, a new meroditerpene from an Indonesian sponge Haliclona sp  Chem Pharm Bull (Tokyo)  2011  59(10):1311-3  PubMed ID: 21963646   DOI: 10.1248/cpb.59.1311

19337  Aratake S, Trianto A, Hanif N, de Voogd NJ, Tanaka J.  A new polyunsaturated brominated fatty acid from a Haliclona sponge  Mar Drugs  2009  7(4):523-7  PubMed ID: 20098595   DOI: 10.3390/md7040523

16072  Nagasaki A, Inotsume K, Kanada M, Uyeda TQ.  Phospholipase D is essential for keratocyte-like migration of NBT-II cells  Cell Struct Funct  2008  33(1):27-33  PubMed ID: 18344600   DOI: 10.1247/csf.07042