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細胞番号 : 細胞名
RCB1366 : HuH-7  update : 2021/02/26
細胞特性(Comment:英)Said producing alpha-fetoprotein, alpha-antitrypsin, ceruloplasmin, fibrinogen, fibronectin, etc.
細胞特性(日)日本人肝癌。アルブミン、alpha-fetoprotein、alpha-antitrypsin、ceruloplasmin、fibrinogen、fibronectin、haptoglobin、transferrin等を産生すると言われている。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
使用条件(日)営利目的か非営利目的かを問わず、いかなる使用につきましても、承諾者(寄託者)から承諾を得ることが必要です。
備考(英)
備考(日)
承諾者住所
×
Japanese
住所
700-8558 岡山県岡山市北区鹿田町2-5-1
岡山大学   医歯薬学総合研究科 細胞生物学分野
阪口 政清 先生
Fax. 086-235-7400

English
Address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.Sakaguchi, Masakiyo
Fax. +81-86-235-7400
提供申込書類(英) Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   承諾書C-0002.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Huh, Nam, Ho
樹立者 Nakabayashi, Hidekazu
寄託日 1997
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Male
57 years
採取組織 liver
病名 Hepatoma
細胞分類 cancer
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 100U/ml Penicillin + 100μg/ml Streptomycin
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 1-2 times/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HBV) (-)
ウィルス(HCV) (-)
アイソザイム検査 LD, NP, MPI, PepB
染色体検査 105-114(50) : 105(6),106(8),107(8),109(4),110(6)/
文献情報 Reference(英) 3件
Reference(日) 2件
利用者成果(英) 30件
利用者成果(日) 0件

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Reference(英)
3427  H Nakabayashi, K Taketa, T Yamane, M Oda, J Sato  Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines p  Cancer Res  1985  45(12 Pt 1):6379-83  PubMed ID: 2415243
2578  Nakabayashi H, Taketa K, Yamane T, Miyazaki M, Miyano K, Sato J  Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.  Gann  1984  75:151-8  PubMed ID: 6203805
135  Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J  Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.  Cancer Res  1982  42:3858-63  PubMed ID: 6286115

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Reference(日)
3449  中林 秀和, 武田 和久  高分化型ヒト肝癌由来細胞株“HuH-7”  岡山医学会雑誌  2012  124:231-238 
2579  Matuda, Kouichi  ヒト肝癌:HuH-7  生体の科学  1992  43:382 

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利用者成果(英)
20081  Tokuda K, Morine Y, Miyazaki K, Yamada S, Saito Y, Nishi M, Tokunaga T, Ikemoto T, Imura S, Shimada M.  The interaction between cancer associated fibroblasts and tumor associated macrophages via the osteopontin pathway in the tumor microenvironment of hepatocellular carcinoma.  Oncotarget  2021  12(4):333-343  PubMed ID: 33659044
13454  Wataru Matsunaga, Misa Ichikawa, Takahiro Ishikawa, Akinobu Gotoh  Lentiviral vector-mediated transfection of human cancer cell lines with tumor suppressor genes inhibits proliferation in vitro  Personalized Medicine Universe  2020/1/1   
12072  Yu Q, Dai J, Shu M.  Retraction: Hsa_circ_0003645 shows an oncogenic role by sponging microRNA-1299 in hepatocellular carcinoma cells.  J. Clin. Lab. Anal.  2020    PubMed ID: 32108372
13377  Feng R, Cui Z, Liu Z, Zhang Y.  Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro.  Scand J Immunol  2020    PubMed ID: 33264420
13400  Yoshinaga N, Uchida S, Dirisala A, Naito M, Osada K, Cabral H, Kataoka K.  mRNA loading into ATP-responsive polyplex micelles with optimal density of phenylboronate ester crosslinking to balance robustness in the biological milieu and intracellular translational efficiency.  J Control Release  2020    PubMed ID: 33359053
13537  Wang T, Jin H, Hu J, Li X, Ruan H, Xu H, Wei L, Dong W, Teng F, Gu J, Qin W, Luo X, Hao Y.  COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling  J Exp Clin Cancer Res  2020  39(1):148  PubMed ID: 32746865
10097  Zhang Y, Zhang LX, Liu XQ, Zhao FY, Ge C, Chen TY, Yao M, Li JJ.  Id4 promotes cell proliferation in hepatocellular carcinoma.  Chin J Cancer  2017  36:19  PubMed ID: 28143562
10284  Singh S, Anupriya MG, Sreekumar E.  Comparative whole genome analysis of dengue virus serotype-2 strains differing in trans-endothelial cell leakage induction in vitro.  Infect. Genet. Evol.  2017  52:34-43  PubMed ID: 28456663
9125  Sreeja R Nair, Rachy Abraham, Sankar Sundaram, Easwaran Sreekumar  Interferon Regulated Gene (IRG) Expression-Signature in a Mouse Model of Chikungunya Virus Neurovirulence  J Neurovirol   2017  23 (6), 886-902  PubMed ID: 29067635
12651  Zhang Y, Tao X, Jin G, Jin H, Wang N, Hu F, Luo Q, Shu H, Zhao F, Yao M, Fang J, Cong W, Qin W, Wang C.  Aggressiveness in Hepatocellular Carcinoma  Theranostics  2016  6(4):558-70  PubMed ID: 26941848
12537  Fujita K, Iwama H, Sakamoto T, Okura R, Kobayashi K, Takano J, Katsura A, Tatsuta M, Maeda E, Mimura S, Nomura T, Tani J, Miyoshi H, Morishita A, Yoneyama H, Yamana Y, Himoto T, Okano K, Suzuki Y, Niki T, Hirashima M, Masaki T.  Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo  Int J Oncol  2015  46(6):2419-30  PubMed ID: 25823465
13709  Marquardt JU, Gomez-Quiroz L, Arreguin Camacho LO, Pinna F, Lee YH, Kitade M, Domínguez MP, Castven D, Breuhahn K, Conner EA, Galle PR, Andersen JB, Factor VM, Thorgeirsson SS.  Curcumin effectively inhibits oncogenic NF-κB signaling and restrains stemness features in liver cancer  J Hepatol  2015  63(3):661-9.  PubMed ID: 25937435
6329  Wang H, Wang B, Liao Q, An H, Li W, Jin X, Cui S, Zhao L.  Overexpression of RhoGDI, a novel predictor of distant metastasis, promotes cell proliferation and migration in hepatocellular carcinoma.  FEBS Lett  2014  588(3):503-8  PubMed ID: 24374343
5844  Kanno T, Tanaka A, Shimizu T, Nakano T, Nishizaki T.  1-[2-(2-Methoxyphenylamino)ethylamino]-3-(naphthalene-1-yloxy)propan-2-ol as a potential anticancer drug.  Pharmacology  2013  91(5-6):339-45  PubMed ID: 23817168
6321  Wang H, Li W, Jin X, Cui S, Zhao L.  LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma.  Eur J Cancer. 2013   2013  49(4):974-83  PubMed ID: 23084841
12720  Hagiwara K, Nakata M, Koyama Y, Sato T.  The effects of coating pDNA/chitosan complexes with chondroitin sulfate on physicochemical characteristics and cell transfection  Biomaterials  2012  33(29):7251-60  PubMed ID: 22796168
13722  Pan K, Liang XT, Zhang HK, Zhao JJ, Wang DD, Li JJ, Lian Q, Chang AE, Li Q, Xia JC.  Characterization of bridging integrator 1 (BIN1) as a potential tumor suppressor and prognostic marker in hepatocellular carcinoma  Mol Med  2012  18(1):507-18  PubMed ID: 22281836
7718  Okudaira N, Goto M, Yanobu-Takanashi R, Tamura M, An A, Abe Y, Kano S, Hagiwara S, Ishizaka Y, Okamura T.  Involvement of retrotransposition of long interspersed nucleotide element-1 in skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate.  Cancer Sci.  2011  102:2000-6  PubMed ID: 21827582
7762  Kawashima R, Abei M, Fukuda K, Nakamura K, Murata T, Wakayama M, Seo E, Hasegawa N, Mizuguchi H, Obata Y, Hyodo I, Hamada H, Yokoyama KK.  EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.  Int. J. Cancer  2011  129:1244-53  PubMed ID: 21710497
7896  Goto K, Tatsumi K, Nagata T, Komizu Y, Ueoka R.  Specific interaction between Ca2+ and anionic lipids in cancer cells along with apoptosis.  Biol. Pharm. Bull.  2011  34:439-42  PubMed ID: 21372400
8252  Ma H, Liang X, Chen Y, Pan K, Sun J, Wang H, Wang Q, Li Y, Zhao J, Li J, Chen M, Xia J.  Decreased expression of BATF2 is associated with a poor prognosis in hepatocellular carcinoma.  Int. J. Cancer  2011  128:771-7  PubMed ID: 20473897
8132  Tjeldhorn L, Iversen N, Sandvig K, Bergan J, Sandset PM, Skretting G.  Functional characterization of the protein C A267T mutation: evidence for impaired secretion due to defective intracellular transport.  BMC Cell Biol.  2010  11:67  PubMed ID: 20815936
8467  Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
8737  Suzuki T, Sakurai F, Nakamura S, Kouyama E, Kawabata K, Kondoh M, Yagi K, Mizuguchi H.  miR-122a-regulated expression of a suicide gene prevents hepatotoxicity without altering antitumor effects in suicide gene therapy.  Mol. Ther.  2008  16:1719-26  PubMed ID: 18665157
8798  Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
8828  Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
6505  Ikeda T, Saito T, Ushita M, Yano F, Kan A, Itaka K, Moro T, Nakamura K, Kawaguchi H, Chung UI.  Identification and characterization of the human SOX6 promoter.  Biochem Biophys Res Commun  2007  357(2):383-90  PubMed ID: 17433257
3270  Inamura J, Ikuta K, Jimbo J, Shindo M, Sato K, Torimoto Y, Kohgo Y.  Upregulation of hepcidin by interleukin-1beta in human hepatoma cell lines.  Hepatol. Res.  2005  33:198-205  PubMed ID: 16198622
9400  Ikeda T, Zhang J, Chano T, Mabuchi A, Fukuda A, Kawaguchi H, Nakamura K, Ikegawa S.  Identification and characterization of the human long form of Sox5 (L-SOX5) gene.  Gene  2002  298:59-68  PubMed ID: 12406576
3784  Wang J, Zhang PC, Lu HF, Ma N, Wang S, Mao HQ, Leong KW.  New polyphosphoramidate with a spermidine side chain as a gene carrier.  J Control Release  2002  83(1):157-68  PubMed ID: 12220847

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