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Cell No. : Cell Name
RCB1366 : HuH-7  update : 2018/07/06
CommentSaid producing alpha-fetoprotein, alpha-antitrypsin, ceruloplasmin, fibrinogen, fibronectin, etc.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.Sakaguchi, Masakiyo
Fax. +81-86-235-7400
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Huh, Nam, Ho
Originator Nakabayashi, Hidekazu
Year of deposit 1997
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 57 years
Tissue liver
Case history Hepatoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics 100U/ml Penicillin + 100μg/ml Streptomycin
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 105-114(50) : 105(6),106(8),107(8),109(4),110(6)/
Reference information Reference 0
User's Publication 20

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User's Publication
12072  Yu Q, Dai J, Shu M.  Retraction: Hsa_circ_0003645 shows an oncogenic role by sponging microRNA-1299 in hepatocellular carcinoma cells.  J. Clin. Lab. Anal.  2020    PubMed ID: 32108372
10097  Zhang Y, Zhang LX, Liu XQ, Zhao FY, Ge C, Chen TY, Yao M, Li JJ.  Id4 promotes cell proliferation in hepatocellular carcinoma.  Chin J Cancer  2017  36:19  PubMed ID: 28143562
10284  Singh S, Anupriya MG, Sreekumar E.  Comparative whole genome analysis of dengue virus serotype-2 strains differing in trans-endothelial cell leakage induction in vitro.  Infect. Genet. Evol.  2017  52:34-43  PubMed ID: 28456663
9125  Sreeja R Nair, Rachy Abraham, Sankar Sundaram, Easwaran Sreekumar  Interferon Regulated Gene (IRG) Expression-Signature in a Mouse Model of Chikungunya Virus Neurovirulence  J Neurovirol   2017  23 (6), 886-902  PubMed ID: 29067635
6329  Wang H, Wang B, Liao Q, An H, Li W, Jin X, Cui S, Zhao L.  Overexpression of RhoGDI, a novel predictor of distant metastasis, promotes cell proliferation and migration in hepatocellular carcinoma.  FEBS Lett  2014  588(3):503-8  PubMed ID: 24374343
5844  Kanno T, Tanaka A, Shimizu T, Nakano T, Nishizaki T.  1-[2-(2-Methoxyphenylamino)ethylamino]-3-(naphthalene-1-yloxy)propan-2-ol as a potential anticancer drug.  Pharmacology  2013  91(5-6):339-45  PubMed ID: 23817168
6321  Wang H, Li W, Jin X, Cui S, Zhao L.  LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma.  Eur J Cancer. 2013   2013  49(4):974-83  PubMed ID: 23084841
7718  Okudaira N, Goto M, Yanobu-Takanashi R, Tamura M, An A, Abe Y, Kano S, Hagiwara S, Ishizaka Y, Okamura T.  Involvement of retrotransposition of long interspersed nucleotide element-1 in skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate.  Cancer Sci.  2011  102:2000-6  PubMed ID: 21827582
7762  Kawashima R, Abei M, Fukuda K, Nakamura K, Murata T, Wakayama M, Seo E, Hasegawa N, Mizuguchi H, Obata Y, Hyodo I, Hamada H, Yokoyama KK.  EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.  Int. J. Cancer  2011  129:1244-53  PubMed ID: 21710497
7896  Goto K, Tatsumi K, Nagata T, Komizu Y, Ueoka R.  Specific interaction between Ca2+ and anionic lipids in cancer cells along with apoptosis.  Biol. Pharm. Bull.  2011  34:439-42  PubMed ID: 21372400
7948  Yang D, Yaguchi T, Nagata T, Gotoh A, Dovat S, Song C, Nishizaki T.  AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis.  Cell. Physiol. Biochem.  2011  27:37-44  PubMed ID: 21325820
8132  Tjeldhorn L, Iversen N, Sandvig K, Bergan J, Sandset PM, Skretting G.  Functional characterization of the protein C A267T mutation: evidence for impaired secretion due to defective intracellular transport.  BMC Cell Biol.  2010  11:67  PubMed ID: 20815936
8252  Ma H, Liang X, Chen Y, Pan K, Sun J, Wang H, Wang Q, Li Y, Zhao J, Li J, Chen M, Xia J.  Decreased expression of BATF2 is associated with a poor prognosis in hepatocellular carcinoma.  Int. J. Cancer  2010  128:771-7  PubMed ID: 20473897
8467  Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
8737  Suzuki T, Sakurai F, Nakamura S, Kouyama E, Kawabata K, Kondoh M, Yagi K, Mizuguchi H.  miR-122a-regulated expression of a suicide gene prevents hepatotoxicity without altering antitumor effects in suicide gene therapy.  Mol. Ther.  2008  16:1719-26  PubMed ID: 18665157
8798  Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
8828  Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
6505  Ikeda T, Saito T, Ushita M, Yano F, Kan A, Itaka K, Moro T, Nakamura K, Kawaguchi H, Chung UI.  Identification and characterization of the human SOX6 promoter.  Biochem Biophys Res Commun  2007  357(2):383-90  PubMed ID: 17433257
3270  Inamura J, Ikuta K, Jimbo J, Shindo M, Sato K, Torimoto Y, Kohgo Y.  Upregulation of hepcidin by interleukin-1beta in human hepatoma cell lines.  Hepatol. Res.  2005  33:198-205  PubMed ID: 16198622
9400  Ikeda T, Zhang J, Chano T, Mabuchi A, Fukuda A, Kawaguchi H, Nakamura K, Ikegawa S.  Identification and characterization of the human long form of Sox5 (L-SOX5) gene.  Gene  2002  298:59-68  PubMed ID: 12406576

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