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Cell No. : Cell Name
RCB1317 : KU-SN  update : 2022/04/01
CommentRare peripheral neuroectodermal tumor cells. Use collagen-coated dish.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Sano Children's Clinic

3-4-20 koyama, nishi-ku, kobe, Hyogo, 651-2144, Japan
Dr.SANO Kimihiko
Fax. +81-78-926-1503
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sano, Kimihiko
Originator Sano, Kimihiko
Year of deposit 1997
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 4 years
Tissue scapula, pleural fluid
Disease name peripheral neuroectodermal
Metastatic ability Yes
Metastatic tissue lung, pleura
Classification cancer
Year of origin 1991
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_8730
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Chromosome mode 69-76(50) : 69(1),70(8),71(6),72(10),73(16),74(7)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
2207  Matsui T, Sano K, Tsukamoto T, Ito M, Takaishi T, Nakata H, Nakamura H, Chihara K  Human neuroblastoma cells express alpha and beta platelet-derived growth factor receptors coupling with neurotrophic and chemotactic signaling.  J Clin Invest  1993  92:1153-60  PubMed ID: 8376577   DOI: 10.1172/JCI116684
2206  Goji J, Sano K, Nakamura H, Ito H  Chondrocytic differentiation of peripheral neuroectodermal tumor cell line in nude mouse xenograft.  Cancer Res  1992  52:4214-20  PubMed ID: 1379122  

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