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Cell No. : Cell Name
RCB1316 : CTB-1  update : 2022/07/22
CommentFas-antigen expressing. Ligation with anti-Fas mAb does not induce apoptosis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyazawa, Keisuke
Originator Miyazawa, Keisuke & Uchida, Yoshiko
Year of deposit 1997
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 70 years
Tissue lymph node, B-lymphoma
Disease name non-Hodgkin's lymphoma
Metastastatic ability Yes
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1149
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Virus (EBV) (+)
Isozyme LD, NP
Chromosome mode 47-49(50) : 47(1),48(48),49(1)
STR(human) OK
Reference information Reference 1
User's Publication 1


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Reference
2176    Establishment of a novel B-lymphoma cell line, CTB-1, with strong Fas antigen expression having chromosomal translocation (14:22)  Int J Oncol  1997  10:1103-1107  PubMed ID: 21533490   DOI: 10.3892/ijo.10.6.1103

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User's Publication
14304  Tahara K, Takizawa M, Yamane A, Osaki Y, Ishizaki T, Mitsui T, Yokohama A, Saitoh T, Tsukamoto N, Matsumoto M, Murakami H, Nojima Y, Handa H.  Overexpression of B-cell lymphoma 6 alters gene expression profile in a myeloma cell line and is associated with decreased DNA damage response  Cancer Sci  2017  108(8):1556-1564  PubMed ID: 28544233   DOI: 10.1111/cas.13283



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