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Cell No. : Cell Name
RCB1312 : C57BL/6J-emb  update : 2022/09/07
CommentMouse(C57BL/6J) embryonic fibroblasts cultured under the 3T3 schedule.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Saijo, Kaoru
Year of deposit 1997
Animal mouse < Mammals
Strain name C57BL/6J
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Year of origin 1997
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5U84
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 2

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User's Publication
8091  Kubo E, Yoshikawa N, Kunitomo M, Kagota S, Shinozuka K, Nakamura K.  Inhibitory effect of Cordyceps sinensis on experimental hepatic metastasis of melanoma by suppressing tumor cell invasion.  Anticancer Res.  2010  30:3429-33  PubMed ID: 20944118  
2555  Nakamura, Kazuki, Yoshikawa, Noriko, Yamaguchi, Yu, Kagota, Satomi, Shinozuka, Kazumasa, Kunitomo, Masaru  Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.  Life Sci  2002  70:791-8  PubMed ID: 11833741   DOI: 10.1016/s0024-3205(01)01454-0

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