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Cell No. : Cell Name
RCB1304 : anti-αB-crystallin  update : 2022/04/01
CommentAnti-chick αB-crystallin MoAb producing. Bacteria(+), therefore, must add penicillin+streptomycin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kosaka, Mitsuko
Originator Sawada, Kaichiro
Year of deposit 1997
Another name anti-αB, 2D2B6
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U813
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Female
Fusion_cell_2 name P3-X63-Ag8-U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name anti-αB-crystallin
Subclass IgG
Immunogen chicken lens
Antigen_name αB-crystallin
Antigen_m.w. 20, 21kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium DMEM/HamF12 + 10% FBS
Antibiotics 100U/ml Penicillin + 50μg/ml Streptomycin
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
deposit info
lot info
Reference information Reference 3
User's Publication 4

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2175  Sawada K, Agata K, Yoshiki A, Eguchi G  A set of anti-crystallin monoclonal antibodies for detecting lens specificities: beta-crystallin as a specific marker for detecting lentoidogenesis in cultures of chicken lens epithelial cells.  Jpn J Ophthalmol  1993  37:355-68  PubMed ID: 7832826  
11033  K Agata, H Kobayashi, Y Itoh, M Mochii, K Sawada, G Eguchi  Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro  Development  1993  118(4):1025-30  PubMed ID: 8269838   DOI: 10.1242/dev.118.4.1025
10638  K Sawada, K Agata, G Eguchi  Crystallin gene expression in the process of lentoidogenesis in cultures of chicken lens epithelial cells  Exp Eye Res  1992  55(6):879-87  PubMed ID: 1283129   DOI: 10.1016/0014-4835(92)90014-j

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User's Publication
15092  van de Schootbrugge C, van Asten F, Nagtegaal ID, Versleijen-Jonkers YM, van Laarhoven HW, Roeffen MH, Sweep FC, Bussink J, Kaanders JH, Pruijn GJ, Boelens WC, Span PN.  αB-crystallin expression is correlated with phospho-ERK1/2 expression in human breast cancer  Int J Biol Markers  2013  28(4):e365-70  PubMed ID: 23722303   DOI: 10.5301/jbm.5000032
15094  Wilhelmus MM, Boelens WC, Kox M, Maat-Schieman ML, Veerhuis R, de Waal RM, Verbeek MM.  Small heat shock proteins associated with cerebral amyloid angiopathy of hereditary cerebral hemorrhage with amyloidosis (Dutch type) induce interleukin-6 secretion  Neurobiol Aging  2009  30(2):229-40  PubMed ID: 17629591   DOI: 10.1016/j.neurobiolaging.2007.06.001
2737  den Engelsman, John, Bennink, Erik J, Doerwald, Linda, Onnekink, Carla, Wunderink, Lisa, Andley, Usha P, Kato, Kanefusa, de Jong, Wilfried W, Boelens, Wilbert C  Mimicking phosphorylation of the small heat-shock protein alphaB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles.  Eur J Biochem  2004  271:4195-203  PubMed ID: 15511225   DOI: 10.1111/j.1432-1033.2004.04359.x
15625  van Rijk AE, Stege GJ, Bennink EJ, May A, Bloemendal H.  Nuclear staining for the small heat shock protein alphaB-crystallin colocalizes with splicing factor SC35  Eur J Cell Biol   2003  82(7):361-8  PubMed ID: 12924631   DOI: 10.1078/0171-9335-00321

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