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Cell No. : Cell Name
RCB1303 : anti-αA-crystallin  update : 2022/04/01
CommentAnti-chick αA-crystallin MoAb producing. Bacteria(+), therefore, must add penicillin+streptomycin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kosaka, Mitsuko
Originator Sawada Kaichiro
Year of deposit 1997
Another name anti-αA
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U830
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Female
Fusion_cell_2 name P3-X63-Ag8-U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name anti-αA-crystallin
Subclass IgG
Immunogen chicken lens
Antigen_name αA-crystallin(mouse,chicken,newt,human)
Antigen_m.w. 20, 21kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics 100U/ml Penicillin + 50μg/ml Streptomycin
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
2175  Sawada K, Agata K, Yoshiki A, Eguchi G  A set of anti-crystallin monoclonal antibodies for detecting lens specificities: beta-crystallin as a specific marker for detecting lentoidogenesis in cultures of chicken lens epithelial cells.  Jpn J Ophthalmol  1993  37:355-68  PubMed ID: 7832826  
11033  K Agata, H Kobayashi, Y Itoh, M Mochii, K Sawada, G Eguchi  Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro  Development  1993  118(4):1025-30  PubMed ID: 8269838  
10638  K Sawada, K Agata, G Eguchi  Crystallin gene expression in the process of lentoidogenesis in cultures of chicken lens epithelial cells  Exp Eye Res  1992  55(6):879-87  PubMed ID: 1283129   DOI: 10.1016/0014-4835(92)90014-j

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