Cell No. : Cell Name
RCB1303 : anti-αA-crystallin
update : 2024/01/17
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| Comment | Anti-chick αA-crystallin MoAb producing. Bacteria(+), therefore, must add penicillin+streptomycin. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kosaka, Mitsuko
|
| Originator |
Sawada Kaichiro
|
| Year of deposit |
1997
|
| Another name |
anti-αA
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_U830
|
|
hybridoma
|
Fusion_cell_1 name |
spleen
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_1 gender |
Female
|
| Fusion_cell_2 name |
P3-X63-Ag8-U1
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| Fusion_cell_2 strain |
mouse, BALB/c
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|
Fusion method |
PEG, HAT selection
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|
Antibody name |
anti-αA-crystallin
|
| Subclass |
IgG
|
| Immunogen |
chicken lens
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|
Antigen_name |
αA-crystallin(mouse,chicken,newt,human)
|
| Antigen_m.w. |
20, 21kDa
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
DMEM/HamF12 + 10% FBS
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| Antibiotics |
|
100U/ml Penicillin + 50μg/ml Streptomycin
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2175
Sawada K, Agata K, Yoshiki A, Eguchi G
A set of anti-crystallin monoclonal antibodies for detecting lens specificities: beta-crystallin as a specific marker for detecting lentoidogenesis in cultures of chicken lens epithelial cells.
Jpn J Ophthalmol
1993
37:355-68
PubMed ID: 7832826
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11033
K Agata, H Kobayashi, Y Itoh, M Mochii, K Sawada, G Eguchi
Genetic characterization of the multipotent dedifferentiated state of pigmented epithelial cells in vitro
Development
1993
118(4):1025-30
PubMed ID: 8269838
DOI: 10.1242/dev.118.4.1025
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10638
K Sawada, K Agata, G Eguchi
Crystallin gene expression in the process of lentoidogenesis in cultures of chicken lens epithelial cells
Exp Eye Res
1992
55(6):879-87
PubMed ID: 1283129
DOI: 10.1016/0014-4835(92)90014-j
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