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Cell No. : Cell Name
RCB1297 : HYT-1  update : 2022/03/31
CommentA human cell line derived from acute myeloid leukemia (AML). Secondary AML. Able to differentiate to megakaryocyte-like cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hamaguchi, Hiroyuki
Originator Hamaguchi, Hiroyuki
Year of deposit 1997
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 62 years
Tissue bone marrow
Disease name AML
Classification cancer
Year of origin 1993
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4639
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated) + 10ng/ml human G-CSF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP, G6PD
Chromosome mode 44-47(50) : 44(5),45(40),46(4),47(1)
STR(human) OK
Images
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lot info
Reference information Reference 0
User's Publication 0


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