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Cell No. : Cell Name
RCB1294 : HRC-17  update : 2022/03/31
CommentHybridoma producing anti-E antigen of the human Rh blood group system
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Nihon Yuketsu Gakkaishi 1992;38:446-452, Nihon Yuketsu Gakkaishi 1993;39:840-846) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sekiguchi, Sadayoshi
Originator Sekiguchi, Sadayoshi
Year of deposit 1996
Another name E-17-41-23-22
Original cell E17
Animal human x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C474
hybridoma Fusion_cell_1 name EBV transformed human lymphocytes
Fusion_cell_1 strain human
Fusion_cell_2 name P3x63Ag8.653
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Subclass human IgM
Immunogen Rh blood group E
Antigen_name Rh(E)
Antigen_m.w. 30〜32kDa
Antigen_positive E-positive red cell
Antigen_negative E-negative red cell
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives GIT
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Reference information Reference 0
User's Publication 0


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