Cell No. : Cell Name
RCB1294 : HRC-17
update : 2024/01/17
|
| Comment | Hybridoma producing anti-E antigen of the human Rh blood group system |
| Comment from the depositor | |
| Terms and conditions | No restriction, but required to cite ref. (Nihon Yuketsu Gakkaishi 1992;38:446-452, Nihon Yuketsu Gakkaishi 1993;39:840-846) and to send a reprint to originator. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sekiguchi, Sadayoshi
|
| Originator |
Sekiguchi, Sadayoshi
|
| Year of deposit |
1996
|
| Another name |
E-17-41-23-22
|
| Original cell |
E17
|
| Animal |
_human x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_C474
|
|
hybridoma
|
Fusion_cell_1 name |
EBV transformed human lymphocytes
|
| Fusion_cell_1 strain |
human
|
| Fusion_cell_2 name |
P3x63Ag8.653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG, HAT selection
|
|
Subclass |
human IgM
|
| Immunogen |
Rh blood group E
|
|
Antigen_name |
Rh(E)
|
| Antigen_m.w. |
30〜32kDa
|
|
Antigen_positive |
E-positive red cell
|
| Antigen_negative |
E-negative red cell
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture medium |
|
GIT
|
| Culture method |
|
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 5 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
GIT : 2 x FM1 = 1 : 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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