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Cell No. : Cell Name
RCB1290 : OIH-1  update : 2022/03/31
CommentA human cell line derived from acute myeloid leukemia (AML). AML was originated from MDS. Possessing a mutation in DCC gene. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hamaguchi, Hiroyuki
Originator Hamaguchi, Hiroyuki
Year of deposit 1996
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 64 years
Tissue blood
Disease name myeloid leukemia
Classification cancer
Year of origin 2001
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4872
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated) + 20ng/ml human G-CSF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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2334  Hamaguchi H, Inokuchi K, Nara N, Nagata K, Yamamoto K, Yagasaki F, Dan K  Alterations in the colorectal carcinoma gene and protein in a novel human myeloid leukemia cell line with trisomy 18 established from overt leukemia after myelodysplastic syndrome.  Int J Hematol  1998  67:153-64  PubMed ID: 9631582  

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User's Publication
2974  Tamura H, Dan K, Tamada K, Nakamura K, Shioi Y, Hyodo H, Wang SD, Dong H, Chen L, Ogata K  Expression of functional B7-H2 and B7.2 costimulatory molecules and their prognostic implications in de novo acute myeloid leukemia  Clin Cancer Res  2005  15:5708-5717  PubMed ID: 16115907   DOI: 10.1158/1078-0432.CCR-04-2672

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