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Cell No. : Cell Name
RCB1287 : ECPC-4  update : 2022/09/13
CommentChoroid plexus carcinoma cell line transfected with IgH enhancer-SV40 T fusion gene. Different shape
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Enjoji, Munechika
Originator Enjoji, Munechika
Year of deposit 1996
Animal mouse < Mammals
Strain name C57BL/6
Tissue choroid
Disease name choroid plexus carcinoma
Classification cancer
Recombinant recombinant
Exogene SV40 T antigen
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4836
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 5


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Reference
2345  Enjoji M, Iwaki T, Hara H, Sakai H, Nawata H, Watanabe T  Establishment and Characterization of Choroid Plexus Carcinoma Cell Lines : Connection Between Choroid Plexus and Immune Systems  Jpn J Cancer Res  1996  87:893-899  PubMed ID: 8878450  
2344  Enjoji M, Iwaki T, Nawata H, Watanabe T  IgH intronic enhancer element HE2 (mu B) functions as a cis-activator in choroid plexus cells at the cellular level as well as in transgenic mice.  J Neurochem  1995  64:961-6  PubMed ID: 7861178  

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User's Publication
12053  Krishnan M, Hwang JS, Kim M, Kim YJ, Seo JH, Jung J, Ha E.  β-hydroxybutyrate Impedes the Progression of Alzheimer's Disease and Atherosclerosis in ApoE-Deficient Mice.  Nutrients  2020    PubMed ID: 32069870   DOI: 10.3390/nu12020471
9537  Takano M, Otani M, Kaji T, Sano K, Hamada-Kanazawa M, Matsuyama S.  Proteomic analysis of mouse choroid plexus cell line ECPC-4 treated with lipid A.  Inflamm. Res.  2016  65:295-302  PubMed ID: 26794622   DOI: 10.1007/s00011-016-0916-3
12910  Lazarevic I, Engelhardt B.  Modeling immune functions of the mouse blood-cerebrospinal fluid barrier in vitro: primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier  Fluids Barriers CNS  2016  13:2  PubMed ID: 26833402   DOI: 10.1186/s12987-016-0027-0
7259  Takano M, Ohkusa M, Otani M, Min KS, Kadoyama K, Minami K, Sano K, Matsuyama S.  Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells.  Immunol. Lett.  2015  167:57-62  PubMed ID: 26235132   DOI: 10.1016/j.imlet.2015.07.007
2872  Watanabe H, Seino T, Sato Y  Antibodies to triosephosphate isomerase in patients with neuropsychiatric lupus.  Biochem Biophys Res Commun  2004  321:949-53  PubMed ID: 15358119   DOI: 10.1016/j.bbrc.2004.07.051



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