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Cell No. : Cell Name
RCB1286 : ECPC-3  update : 2022/03/31
CommentChoroid plexus carcinoma cell line transfected with IgH enhancer-SV40 T fusion gene.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Enjoji, Munechika
Originator Enjoji, M. & Nakashima, M.
Year of deposit 1996
Original cell ECPC
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 12 weeks
Tissue choroid
Disease name choroid plexus carcinoma
Classification cancer
Recombinant recombinant
Exogene SV40 T antigen
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4835
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2345  Enjoji M, Iwaki T, Hara H, Sakai H, Nawata H, Watanabe T  Establishment and Characterization of Choroid Plexus Carcinoma Cell Lines : Connection Between Choroid Plexus and Immune Systems  Jpn J Cancer Res  1996  87:893-899  PubMed ID: 8878450  
2344  Enjoji M, Iwaki T, Nawata H, Watanabe T  IgH intronic enhancer element HE2 (mu B) functions as a cis-activator in choroid plexus cells at the cellular level as well as in transgenic mice.  J Neurochem  1995  64:961-6  PubMed ID: 7861178  

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