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Cell No. : Cell Name
RCB1285 : 3A21  update : 2022/03/31
CommentAnti-ribonuclease A antibody producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Omasa, Takeshi
Originator Suga, Ken-ichi & Omasa, Takeshi
Year of deposit 1996
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J721
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8-U1
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name anti-Ribonuclease A
Subclass IgG
Immunogen bovine pancreatic ribonuclease A
Antigen_name RNase A
Antigen_m.w. 10000
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives (RPMI1640 : DMEM : HamF12 = 2 : 1 : 1) + 0.875g/L Glucose + 0.36g/L HEPES + 10mg/L Insulin + 20mg/L Transferrin + 2ul/L Monoethanolamine + 0.17μg/L Sodium Selenite + 5g/L BSA
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2-3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 15
User's Publication 3


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Reference
2156  Katakura, Y., Kobayashi, E., Kurokawa, Y., Omasa, T., Fujiyama, K., Suga, K.  Cloning of cDNA and Characterization of Anti-RNase A Monoclonal Antibody 3A21  J Fermentation and Bioengineering  1996  82:312-314 
2155  Omasa, T., Kobayashi, M., Nishikawa, T., Shioya, S., Suga, K., Uemura, S., Kitani, Y., Imamura, Y.  Enhancement of Antibody Production by Growth Factor Addition in Perfusion and Hollow-Fiber Culture Sustems  Biotechnol Bioeng  1995  48:673-680  PubMed ID: 18623537
2164  Omasa, T., Shioya, S., Suga, K.  Cell-Cycle Phase Analysis in Glucose-Limited Continuous Cultivation for the Effective Production of Antibody  Animal Cell Technology : Developments towards the 21st Century  1995  289-294 
2154  Kobayashi M, Kato S, Omasa T, Shioya S, Suga K  Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production.  Cytotechnology  1994  15:51-6  PubMed ID: 7765952
2162  Omasa, T., Amimoto, K., Shibata, S., Kobayashi, M., Shioya, S., Suga, K.  The Flow Cytometory Analysys of Hybridoma Cell-Cycle Phase and Antibody Production in Continuous Cultivation  Better living through innovative biochemical engineering  1994  116-118 
2163  Kobayashi, E., Kumamoto, T., Omasa, T., Fujiyama, K., Shioya, S., Suga, K.  The Analysis of Antigen-Antibody Binding Anti-RNase A Single Chain Fv-3A21  Animal Cell Technology : Basic & Applied Aspects  1994  6:243-247 
2161  Omasa, T., Kobayashi, M., Shioya, S., Suga, K.  Effect of the Cell-Cycle Phase on Hybridoma Culture  Animal Cell Technology : Basic & Applied Aspects  1993  5:534-549 
2152  Omasa, T., Higashiyama, K., Shioya, S., Suga, K.  Effects of Lactate Concentration on Hybridoma Culture in Lactate-Controlled Fed-Batch Operation  Biotechnol Bioeng  1992  39:556-564  PubMed ID: 18600982
2153  Omasa T, Ishimoto M, Higashiyama K, Shioya S, Suga K  The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture.  Cytotechnology  1992  8:75-84  PubMed ID: 1368407
2160  Omasa, T., Ishimoto, M., Higashi, K., Shioya, S., SUga, K.  The Effects of Glutamine Concentration on Growth and Monoclonal Antibody Production in Fed-Batch Operation  Animal Cell Technology : Basic & Applied Aspects  1992  265-270 
2165  Suga, K., Hirasawa, T., Shioya, S.,  Purification of RNase A using an Immuno-Affinity Partition in Aqueous-polymer Two Phase System  Solvent Extraction  1992  1779-1784 
2159  Omasa, T., Kobayashi, M., Ishimoto, M., Higashiyama, K., Shioya, S., Suga, K.  The Effects of Glutamine and Glucose Concentration on Hybridoma Cell Growth and Antibody Productivity  Biochemical Engineering for 2001  1992  323-326 
2157  Omasa, T., Kobayashi, M., Nishikawa, T., Shioya, S., Suga, K., Uemura, S., Imamura, Y.  Hybridoma Culture in the Hollw-fiber System -The Effects of Growth Factor-  Animal Cell Culture and Production of Biologicals  1991  229-236 
2158  Omasa, T., Kobayashi, M., Nishikawa, T., Shioya, S., Suga, K., Uemura, S., Imamura, Y.  Effects of Growth Factors on Hybridoma Culture in the Perfusion System  Animal Cell Culture and Production of Biologicals  1991  237-243 
2166  Omasa, T., Higashiyama, K., Shioya, S., Suga, K.,  Effect of Lactate Concentration on Hybridoma Cell Culture in the Fed-Batch Operation  Animal Cell Culture Technology  1990  105-108 

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User's Publication
12898  Okochi M, Kamiya T, Omasa T, Tanaka M, Honda H.  Rapid Colorimetric Antibody Detection Using a Dual-function Peptide Probe for Silver Nanoparticle Aggregation and Antibody Recognition  Anal Sci  2016  32(1):93-7.  PubMed ID: 26753712
8534  Omasa T, Furuichi K, Iemura T, Katakura Y, Kishimoto M, Suga K.  Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture.  Bioprocess Biosyst Eng  2010  33:117-25  PubMed ID: 19590901
8875  Fujiyama K, Misaki R, Katsura A, Tanaka T, Furukawa A, Omasa T, Seki T.  N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells.  J. Biosci. Bioeng.  2006  101:212-8  PubMed ID: 16716920



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