Cell No. : Cell Name RCB1284 : B16 melanoma/lacZ  update : 2023/11/20
Comment			Mouse melanoma expressing E.coli lac Z. Strongly positive with beta-galactosidase.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hamada, Hirofumi
		Year of deposit	1996
		Another name	B16/lacZ
		Animal	_mouse < Mammals
		Strain name	C57BL/6
		Tissue	skin
		Disease name	melanoma
		Classification	cancer
		Recombinant	recombinant
		Exogene	MFGlacZ
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_L291
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

2131  Nakamura Y, Wakimoto H, Abe J, Kanegae Y, Saito I, Aoyagi M, Hirakawa K, Hamada H  Adoptive immunotherapy with murine tumor-specific T lymphocytes engineered to secrete interleukin 2.  Cancer Res  1994  54:5757-60  PubMed ID: 7525049

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User's Publication

20232  Cupovic J, Ring SS, Onder L, Colston JM, Lütge M, Cheng HW, De Martin A, Provine NM, Flatz L, Oxenius A, Scandella E, Krebs P, Engeler D, Klenerman P, Ludewig B.  Adenovirus vector vaccination reprograms pulmonary fibroblastic niches to support protective inflating memory CD8+ T cells.  Nat Immunol  2021    PubMed ID: 34267375   DOI: 10.1038/s41590-021-00969-3

7651  Arena F, Singh JB, Gianolio E, Stefania R, Aime S.  β-Gal gene expression MRI reporter in melanoma tumor cells. Design, synthesis, and in vitro and in vivo testing of a Gd(III) containing probe forming a high relaxivity, melanin-like structure upon β-Gal enzymatic activation.  Bioconjug. Chem.  2011  22:2625-35  PubMed ID: 22035020   DOI: 10.1021/bc200486j