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Cell No. : Cell Name
RCB1284 : B16 melanoma/lacZ  update : 2022/03/31
CommentMouse melanoma expressing E.coli lac Z. Strongly positive with beta-galactosidase.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hamada, Hirofumi
Year of deposit 1996
Another name B16/lacZ
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Disease name melanoma
Classification cancer
Recombinant recombinant
Exogene MFGlacZ
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_L291
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 2

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2131  Nakamura Y, Wakimoto H, Abe J, Kanegae Y, Saito I, Aoyagi M, Hirakawa K, Hamada H  Adoptive immunotherapy with murine tumor-specific T lymphocytes engineered to secrete interleukin 2.  Cancer Res  1994  54:5757-60  PubMed ID: 7525049  

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User's Publication
20232  Cupovic J, Ring SS, Onder L, Colston JM, Lütge M, Cheng HW, De Martin A, Provine NM, Flatz L, Oxenius A, Scandella E, Krebs P, Engeler D, Klenerman P, Ludewig B.  Adenovirus vector vaccination reprograms pulmonary fibroblastic niches to support protective inflating memory CD8+ T cells.  Nat Immunol  2021    PubMed ID: 34267375   DOI: 10.1038/s41590-021-00969-3
7651  Arena F, Singh JB, Gianolio E, Stefania R, Aime S.  β-Gal gene expression MRI reporter in melanoma tumor cells. Design, synthesis, and in vitro and in vivo testing of a Gd(III) containing probe forming a high relaxivity, melanin-like structure upon β-Gal enzymatic activation.  Bioconjug. Chem.  2011  22:2625-35  PubMed ID: 22035020   DOI: 10.1021/bc200486j

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