Cell No. : Cell Name
RCB1281 : TGBC51TKB
update : 2024/01/11
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Comment | 3 cell lines from different metastases were established from the same patient. See TGBC52TKB. Application consideration |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Todoroki, T. & Ohno, T.
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Originator |
Ohno, Tadao & Yasugi Tomoko
|
Year of deposit |
1996
|
Another name |
YaKa
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
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Race |
Japanese
|
Gender |
Male
|
Age at sampling |
52 years
|
Tissue |
papilla vater, peritoneum meta
|
Metastatic ability |
Yes
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Metastatic tissue |
liver, lymph node
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Classification |
cancer
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Year of origin |
1996
|
|
Lifespan |
infinite
|
Morphology |
epithelial-like
|
Cellosaurus(Expasy) |
CVCL_H694
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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DMEM (low glucose) + 10% FBS
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Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : once/10 days, Medium Renewal : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
|
5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma/Acholeplasma |
|
(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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60-72(50)/63(5),64(8),65(8),66(7),67(8),68(4)/
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STR(human) |
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OK
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
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User's Publication |
1
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Reference |
2326
Ghosh M, Koike N, Tsunoda S, Hirano T, Kaul S, Kashiwagi H, Kawamoto T, Ohkohchi N, Saijo K, Ohno T, Miwa M, Todoroki T.
Characterization and genetic analysis in the newly established human bile duct cancer cell lines
Int J Oncol
2005
26(2):449-56
PubMed ID: 15645130
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2319
Koike N, Todoroki T, Kawamoto T, Yoshida S, Kashiwagi H, Fukao K, Ohno T, Watanabe T
The invasion potentials of human biliary tract carcinoma cell lines: correlation between invasiveness and morphologic characteristics.
Int J Oncol
1998
13:1269-74
PubMed ID: 9824643
DOI: 10.3892/ijo.13.6.1269
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