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Cell No. : Cell Name
RCB1281 : TGBC51TKB  update : 2024/01/11
Comment3 cell lines from different metastases were established from the same patient. See TGBC52TKB. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Todoroki, T. & Ohno, T.
Originator Ohno, Tadao & Yasugi Tomoko
Year of deposit 1996
Another name YaKa
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 52 years
Tissue papilla vater, peritoneum meta
Metastatic ability Yes
Metastatic tissue liver, lymph node
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H694
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 60-72(50)/63(5),64(8),65(8),66(7),67(8),68(4)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 2

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2319  Koike N, Todoroki T, Kawamoto T, Yoshida S, Kashiwagi H, Fukao K, Ohno T, Watanabe T  The invasion potentials of human biliary tract carcinoma cell lines: correlation between invasiveness and morphologic characteristics.  Int J Oncol  1998  13:1269-74  PubMed ID: 9824643   DOI: 10.3892/ijo.13.6.1269

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User's Publication
3738  Yamashita-Kashima Y, Yoshimura Y, Fujimura T, Shu S, Yanagisawa M, Yorozu K, Furugaki K, Higuchi R, Shoda J, Harada N.  Molecular targeting of HER2-overexpressing biliary tract cancer cells with trastuzumab emtansine, an antibody-cytotoxic drug conjugate.  Cancer Chemother Pharmacol.  2019  83(4):659-671  PubMed ID: 30659304   DOI: 10.1007/s00280-019-03768-8
2763  Ghosh, Mila, Koike, Naoto, Tsunoda, Shin-Ichi, Hirano, Takashi, Kaul, Sunil, Kashiwagi, Hironobu, Kawamoto, Toru, Ohkohchi, Nobuhiro, Saijo, Kaoru, Ohno, Tadao, Miwa, Masanao, Todoroki, Takeshi  Characterization and genetic analysis in the newly established human bile duct cancer cell lines.  Int J Oncol  2005  26:449-56  PubMed ID: 15645130  

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