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Cell No. : Cell Name
RCB1279 : TtT/GF  update : 2022/08/18
CommentMouse cell line derived from pituitary tumor (thyrotropin-producing tumor). Pituitary folliculo-stellate-like cells. FGF-dependent cell line. GFAP positive. S-100 positive. Said producing IL-6.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1996
Animal mouse < Mammals
Tissue pituitary
Classification semi-normal
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_S512
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 4

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3325  T Yamasaki, H Fujita, K Inoue, T Fujita, N Yamashita  Regulation of K+ channels by cell contact in a cloned folliculo-stellate cell (TtT/GF)  Endocrinology  1997  138(10):4346-50.  PubMed ID: 9322949   DOI: 10.1210/endo.138.10.5445
3326  U Renner, J Gloddek, E Arzt, K Inoue, G K Stalla  Interleukin-6 is an autocrine growth factor for folliculostellate-like TtT/GF mouse pituitary tumor cells  Exp Clin Endocrinol Diabetes  1997  105(6):345-52  PubMed ID: 9439931   DOI: 10.1055/s-0029-1211777
3328  H Kobayashi, J Fukata, N Murakami, T Usui, O Ebisui, S Muro, I Hanaoka, K Inoue, H Imura, K Nakao  Tumor necrosis factor receptors in the pituitary cells  Brain Res  1997  758(1-2):45-50  PubMed ID: 9203532   DOI: 10.1016/s0006-8993(96)01437-0
3298  Z X Zhang, K Koike, Y Sakamoto, H Jikihara, Y Kanda, K Inoue, K Hirota, A Miyake  Pituitary folliculo-stellate-like cell line produces a cytokine-induced neutrophil chemoattractant  Neuropeptides  1997  31(1):46-51  PubMed ID: 9574837   DOI: 10.1016/s0143-4179(97)90019-6
3420  Chiaki Koyama, Hirokazu Matsumoto, Takafumi Sakai, Katsumi Wakabayashi, Akihiro Ito, Ernest F. Couch, Kinji Inoue  Pituitary Folliculo-Stellate-Like Cells Stimulate Somatotroic Pituitary Tumor Growth in Nude Mice  Endocr Pathol  1995  6(1):67-75  PubMed ID: 12114691   DOI: 10.1007/BF02914990
2111  Matsumoto H, Ishibashi Y, Ohtaki T, Hasegawa Y, Koyama C, Inoue K  Newly established murine pituitary folliculo-stellate-like cell line (TtT/GF) secretes potent pituitary glandular cell survival factors, one of which corresponds to metalloproteinase inhibitor.  Biochem Biophys Res Commun  1993  194:909-15  PubMed ID: 8343172   DOI: 10.1006/bbrc.1993.1907
2112  Matsumoto H, Koyama C, Sawada T, Koike K, Hirota K, Miyake A, Arimura A, Inoue K  Pituitary folliculo-stellate-like cell line (TtT/GF) responds to novel hypophysiotropic peptide (pituitary adenylate cyclase-activating peptide), showing increased adenosine 3',5'-monophosphate and interleukin-6 secretion and cell proliferation.  Endocrinology  1993  133:2150-5  PubMed ID: 8404665   DOI: 10.1210/endo.133.5.8404665
2110  Inoue K, Matsumoto H, Koyama C, Shibata K, Nakazato Y, Ito A  Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor.  Endocrinology  1992  131:3110-6  PubMed ID: 1446645   DOI: 10.1210/endo.131.6.1446645

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User's Publication
11301  Hannen R, Steffani M, Voellger B, Carl B, Wang J, Bartsch JW, Nimsky C.  Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study.  J. Steroid Biochem. Mol. Biol.  2019    PubMed ID: 30439415   DOI: 10.1016/j.jsbmb.2018.11.005
12580  Stella Tran, Ying Wang, Pankaj Lamba, Xiang Zhou, Ulrich Boehm, Daniel J Bernard  The CpG island in the murine foxl2 proximal promoter is differentially methylated in primary and immortalized cells  PLoS One  2013  8(10):e76642  PubMed ID: 24098544   DOI: 10.1371/journal.pone.0076642
12577  T Katayama, M Nakashima, H Kyan, N Murakami, H Kuroda  A role of pituitary adenylate cyclase activating polypeptide (PACAP) as a regulator of paracrine interactions between folliculo-stellate cells and gonadotropes through the control of activin-follistatin interactions  J Vet Med Sci  2000  62(7):731-6  PubMed ID: 10945291   DOI: 10.1292/jvms.62.731
12578  Y Kiriyama, H Tsuchiya, T Murakami, K Satoh, Y Tokumitsu  Calcitonin induces IL-6 production via both PKA and PKC pathways in the pituitary folliculo-stellate cell line  Endocrinology  2000  142(8):3563-9  PubMed ID: 11459804   DOI: 10.1210/endo.142.8.8328

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