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Cell No. : Cell Name
RCB1279 : TtT/GF  update : 2020/09/24
CommentPituitary folliculo-stellate-like cells. GFAP positive and S-100 positive.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1996
Animal mouse < Mammals
Tissue pituitary
Classification semi-normal
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
2111  Matsumoto H, Ishibashi Y, Ohtaki T, Hasegawa Y, Koyama C, Inoue K  Newly established murine pituitary folliculo-stellate-like cell line (TtT/GF) secretes potent pituitary glandular cell survival factors, one of which corresponds to metalloproteinase inhibitor.  Biochem Biophys Res Commun  1993  194:909-15  PubMed ID: 8343172
2112  Matsumoto H, Koyama C, Sawada T, Koike K, Hirota K, Miyake A, Arimura A, Inoue K  Pituitary folliculo-stellate-like cell line (TtT/GF) responds to novel hypophysiotropic peptide (pituitary adenylate cyclase-activating peptide), showing increased adenosine 3',5'-monophosphate and interleukin-6 secretion and cell proliferation.  Endocrinology  1993  133:2150-5  PubMed ID: 8404665
2110  Inoue K, Matsumoto H, Koyama C, Shibata K, Nakazato Y, Ito A  Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor.  Endocrinology  1992  131:3110-6  PubMed ID: 1446645

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User's Publication
11301  Hannen R, Steffani M, Voellger B, Carl B, Wang J, Bartsch JW, Nimsky C.  Effects of anti-estrogens on cell invasion and survival in pituitary adenoma cells: A systematic study.  J. Steroid Biochem. Mol. Biol.  2019    PubMed ID: 30439415



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