Cell No. : Cell Name
RCB1278 : MtT/E
update : 2022/03/31
|
| Comment | Rat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing no pituitary hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Inoue, Kinji
|
| Originator |
Inoue, Kinji
|
| Year of deposit |
1996
|
| Animal |
_rat
< Mammals
|
| Strain name |
F344
|
| Gender |
Female
|
| Tissue |
pituitary
|
| Disease name |
mammotropic pituitary tumor
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_H712
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4-8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
7
|
| User's Publication |
0
|
| Reference |
|
3424
Sukumar Devnath, Taro Kataoka, Kaoru Miura, Mie Kusuda, Kunio Kitamura, Yuko Kumada, Akikazu Mochiduki, Kei Kaneko, Akihito Adachi, Kinji Inoue
Cgr11 encodes a secretory protein involved in cell adhesion
Eur J Cell Biol
2009
88(9):521-9
PubMed ID: 19473726
DOI: 10.1016/j.ejcb.2009.04.003
|
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3422
Kotaro Yokoyama, Chihiro Mogi, Kaoru Miura, Keisuke Kuroda, Kinji Inoue
Somatotropes maintain their immature cells through Insulin-like growth factor I (IGF-I)
Endocr Pathol . Fall 2007;18(3):174-81
2007
18(3):174-81
PubMed ID: 18058266
DOI: 10.1007/s12022-007-0016-6
|
|
3426
Haruo Nogami, Yoshiki Hiraoka, Kinji Inoue, Sadakazu Aiso, Setsuji Hisano
Regulation of 5'-promoter activity of the rat growth hormone and growth hormone-releasing hormone receptor genes in the MtT/S and MtT/E cells
Neuroendocrinology
2006
84(1):31-41
PubMed ID: 17090972
DOI: 10.1159/000096826
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3425
C Mogi, H Goda, K Mogi, A Takaki, K Yokoyama, M Tomida, K Inoue
Multistep differentiation of GH-producing cells from their immature cells
J Endocrinol
2005
184(1):41-50
PubMed ID: 15642781
DOI: 10.1677/joe.1.05870
|
|
3324
Hitomi Fukuro, Chihiro Mogi, Kotaro Yokoyama, Kinji Inoue
Change in expression of basic fibroblast growth factor mRNA in a pituitary tumor clonal cell line
Endocr Pathol
2003
14(2):145-9
PubMed ID: 12858005
DOI: 10.1385/ep:14:2:145
|
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3401
N Fujimoto, H Watanabe, A Ito, K Inoue
Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats
Jpn J Cancer Res
1991
82(12):1436-41
PubMed ID: 1778767
DOI: 10.1111/j.1349-7006.1991.tb01817.x
|
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1289
Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A
Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.
Endocrinology
1990
126:2313-20
PubMed ID: 2184014
DOI: 10.1210/endo-126-5-2313
|
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