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Cell No. : Cell Name
RCB1278 : MtT/E  update : 2022/03/31
CommentRat cell line derived from pituitary tumor. Estrogen-induced tumor. Producing no pituitary hormone. Four cell lines (MtT/E, MtT/S, MtT/SM, MtT/Se) were established simultaneously.Application consideration.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 1996
Animal rat < Mammals
Strain name F344
Gender Female
Tissue pituitary
Disease name mammotropic pituitary tumor
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H712
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 7
User's Publication 0


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Reference
3424  Sukumar Devnath, Taro Kataoka, Kaoru Miura, Mie Kusuda, Kunio Kitamura, Yuko Kumada, Akikazu Mochiduki, Kei Kaneko, Akihito Adachi, Kinji Inoue  Cgr11 encodes a secretory protein involved in cell adhesion  Eur J Cell Biol  2009  88(9):521-9  PubMed ID: 19473726   DOI: 10.1016/j.ejcb.2009.04.003
3422  Kotaro Yokoyama, Chihiro Mogi, Kaoru Miura, Keisuke Kuroda, Kinji Inoue  Somatotropes maintain their immature cells through Insulin-like growth factor I (IGF-I)  Endocr Pathol . Fall 2007;18(3):174-81  2007  18(3):174-81  PubMed ID: 18058266   DOI: 10.1007/s12022-007-0016-6
3426  Haruo Nogami, Yoshiki Hiraoka, Kinji Inoue, Sadakazu Aiso, Setsuji Hisano  Regulation of 5'-promoter activity of the rat growth hormone and growth hormone-releasing hormone receptor genes in the MtT/S and MtT/E cells  Neuroendocrinology  2006  84(1):31-41  PubMed ID: 17090972   DOI: 10.1159/000096826
3425  C Mogi, H Goda, K Mogi, A Takaki, K Yokoyama, M Tomida, K Inoue  Multistep differentiation of GH-producing cells from their immature cells  J Endocrinol   2005  184(1):41-50  PubMed ID: 15642781   DOI: 10.1677/joe.1.05870
3324  Hitomi Fukuro, Chihiro Mogi, Kotaro Yokoyama, Kinji Inoue  Change in expression of basic fibroblast growth factor mRNA in a pituitary tumor clonal cell line  Endocr Pathol  2003  14(2):145-9  PubMed ID: 12858005   DOI: 10.1385/ep:14:2:145
3401  N Fujimoto, H Watanabe, A Ito, K Inoue  Estrogen receptor levels and tumor growth in a series of pituitary clonal cell lines in rats  Jpn J Cancer Res  1991  82(12):1436-41  PubMed ID: 1778767   DOI: 10.1111/j.1349-7006.1991.tb01817.x
1289  Inoue K, Hattori M, Sakai T, Inukai S, Fujimoto N, Ito A  Establishment of a series of pituitary clonal cell lines differing in morphology, hormone secretion, and response to estrogen.  Endocrinology  1990  126:2313-20  PubMed ID: 2184014  

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