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Cell No. : Cell Name
RCB1273 : tsBN269  update : 2022/03/31
CommentTemperature-sensitive mutant of BHK-21 selected after MNNG treatment.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Kyushu University

3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 Japan
Fax. +81-92-642-5435
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishimoto, Takeharu
Originator Nishimoto, Takeharu
Year of deposit 1996
Original cell BHK-21(C-13)
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U469
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, PepB
Reference information Reference 2
User's Publication 0

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2132  Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M  Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells  Somat Cell Mol Genet  1982  8:811-824  PubMed ID: 6891837   DOI: 10.1007/BF01543021
2133  Nishimoto T, Basilico C  Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells  Somat Cell Mol Genet  1978  4:323-340  PubMed ID: 694723   DOI: 10.1007/BF01542846

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