Cell No. : Cell Name
update : 2024/11/14
Comment Temperature-sensitive mutant of BHK-21. Complementation group A. Dye rapidly at 39.5 C.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Kyushu University
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Nishimoto, Takeharu
Originator
Nishimoto, Takeharu
Year of deposit
1996
Original cell
BHK-21(C-13)
Animal
_hamster
< Mammals
Strain name
Syrian
Genus
Mesocricetus
Species
auratus
Tissue
kidney
Classification
mutant
Memo_1
Mycoplasma was eliminated with MC-210.
Lifespan
infinite
Morphology
fibroblast-like
Cellosaurus(Expasy)
CVCL_U468
deposit info lot info
Medium
Medium List
Culture type
Adherent cells
Culture medium
DMEM (high glucose) + 10% CS
Antibiotics
Free
Passage method
0.25% Trypsin
Culture information
Passage ratio
1 : 4 split
SC frequency
Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature
33
℃
CO2 concentration
5
%
Freeze medium
Medium + 10% DMSO
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Animal PCR
OK
Isozyme
LD, NP
Images
deposit info lot info
Reference information
Reference
2
User's Publication
0
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