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Cell No. : Cell Name
RCB1270 : tsBN51  update : 2022/03/31
CommentTemperature-sensitive mutant of BHK-21 with defect in RNA polymerase III subunit.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishimoto, Takeharu
Originator Nishimoto, Takeharu
Year of deposit 1996
Original cell BHK-21(C-13)
Animal hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification mutant
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U471
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 0


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Reference
2147  Jackson, A J, Ittmann M, Pugh, B F  The BN51 protein is a polymerase (Pol)-specific subunit of RNA Pol III which reveals a link between Pol III transcription and pre-rRNA processing.  Mol Cell Biol  1995  15:94-101  PubMed ID: 7799973   DOI: 10.1128/mcb.15.1.94
2132  Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M  Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells  Somat Cell Mol Genet  1982  8:811-824  PubMed ID: 6891837  
2133  Nishimoto T, Basilico C  Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells  Somat Cell Mol Genet  1978  4:323-340  PubMed ID: 694723  

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