Cell No. : Cell Name
RCB1270 : tsBN51
update : 2025/06/18
|
| Comment | Temperature-sensitive mutant of BHK-21 with defect in RNA polymerase III subunit. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishimoto, Takeharu
|
| Originator |
Nishimoto, Takeharu
|
| Year of deposit |
1996
|
| Original cell |
BHK-21(C-13)
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Genus |
Mesocricetus
|
| Species |
auratus
|
| Gender |
Unknown
|
| Tissue |
kidney
|
| Classification |
mutant
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_U471
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| DNA barcoding |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2147
Jackson, A J, Ittmann M, Pugh, B F
The BN51 protein is a polymerase (Pol)-specific subunit of RNA Pol III which reveals a link between Pol III transcription and pre-rRNA processing.
Mol Cell Biol
1995
15:94-101
PubMed ID: 7799973
DOI: 10.1128/mcb.15.1.94
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2132
Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M
Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells
Somat Cell Mol Genet
1982
8:811-824
PubMed ID: 6891837
DOI: 10.1007/BF01543021
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2133
Nishimoto T, Basilico C
Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells
Somat Cell Mol Genet
1978
4:323-340
PubMed ID: 694723
DOI: 10.1007/BF01542846
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