Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1269 : tsBN75  update : 2022/03/31
CommentTemperature-senstive mutant of BHK-21. Complemented with ubiquitin-activating enzyme E1 cDNA.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishimoto, Takeharu
Originator Nishimoto, Takeharu
Year of deposit 1996
Original cell BHK-21(C-13)
Animal hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U474
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, PepB
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


To topTop
Reference
2146  Nishitani H, Goto H, Kaneda S, Yamao F, Seno T, Handley P, Schwartz, A L, Nishimoto T  tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.  Biochem Biophys Res Commun  1992  184:1015-21  PubMed ID: 1575720   DOI: 10.1016/0006-291x(92)90692-e
2132  Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M  Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells  Somat Cell Mol Genet  1982  8:811-824  PubMed ID: 6891837  
2133  Nishimoto T, Basilico C  Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells  Somat Cell Mol Genet  1978  4:323-340  PubMed ID: 694723  

To topTop
User's Publication



Back Back Return Top Page