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Cell No. : Cell Name
RCB1268 : tsBN462  update : 2022/03/31
CommentTemperature-sensitive mutant of BHK-21. Complemented with human X-linked CCG1 gene. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishimoto, Takeharu
Originator Nishimoto, Takeharu
Year of deposit 1996
Original cell BHK-21(C-13)
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Tissue kidney
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U470
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Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, PepB
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Reference information Reference 6
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2135  Nakashima T, Sekiguchi T, Sunamoto H, Yura K, Tomoda S, Go M, Kere J, Schlessinger D, Nishimoto T  Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein.  Gene  1994  141:193-200  PubMed ID: 8163188   DOI: 10.1016/0378-1119(94)90570-3
2144  Hayashida T, Sekiguchi T, Noguchi E, Sunamoto H, Ohba T, Nishimoto T  The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster.  Gene  1994  141:267-70  PubMed ID: 8163200   DOI: 10.1016/0378-1119(94)90583-5
2136  Sekiguchi T, Nohiro Y, Nakamura Y, Hisamoto N, Nishimoto T  The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.  Mol Cell Biol  1991  11:3317-25  PubMed ID: 2038334   DOI: 10.1128/mcb.11.6.3317
2137  Sekiguchi T, Miyata T, Nishimoto T  Molecular cloning of the cDNA of human X chromosomal gene (CCG1) which complements the temperature-sensitive G1 mutants, tsBN462 and ts13, of the BHK cell line.  EMBO J  1988  7:1683-7  PubMed ID: 3169001   DOI: 10.1002/j.1460-2075.1988.tb02996.x
2132  Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M  Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells  Somat Cell Mol Genet  1982  8:811-824  PubMed ID: 6891837   DOI: 10.1007/BF01543021
2133  Nishimoto T, Basilico C  Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells  Somat Cell Mol Genet  1978  4:323-340  PubMed ID: 694723   DOI: 10.1007/BF01542846

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