Cell No. : Cell Name
RCB1268 : tsBN462
update : 2025/06/17
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| Comment | Temperature-sensitive mutant of BHK-21. Complemented with human X-linked CCG1 gene. Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nishimoto, Takeharu
|
| Originator |
Nishimoto, Takeharu
|
| Year of deposit |
1996
|
| Original cell |
BHK-21(C-13)
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Genus |
Mesocricetus
|
| Species |
auratus
|
| Tissue |
kidney
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| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_U470
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose) + 10% CS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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33
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| DNA barcoding |
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OK
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| Isozyme |
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LD, NP, G6PD, PepB
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
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| User's Publication |
0
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| Reference |
|
2135
Nakashima T, Sekiguchi T, Sunamoto H, Yura K, Tomoda S, Go M, Kere J, Schlessinger D, Nishimoto T
Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein.
Gene
1994
141:193-200
PubMed ID: 8163188
DOI: 10.1016/0378-1119(94)90570-3
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2144
Hayashida T, Sekiguchi T, Noguchi E, Sunamoto H, Ohba T, Nishimoto T
The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster.
Gene
1994
141:267-70
PubMed ID: 8163200
DOI: 10.1016/0378-1119(94)90583-5
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2136
Sekiguchi T, Nohiro Y, Nakamura Y, Hisamoto N, Nishimoto T
The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.
Mol Cell Biol
1991
11:3317-25
PubMed ID: 2038334
DOI: 10.1128/mcb.11.6.3317
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2137
Sekiguchi T, Miyata T, Nishimoto T
Molecular cloning of the cDNA of human X chromosomal gene (CCG1) which complements the temperature-sensitive G1 mutants, tsBN462 and ts13, of the BHK cell line.
EMBO J
1988
7:1683-7
PubMed ID: 3169001
DOI: 10.1002/j.1460-2075.1988.tb02996.x
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2132
Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M
Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells
Somat Cell Mol Genet
1982
8:811-824
PubMed ID: 6891837
DOI: 10.1007/BF01543021
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2133
Nishimoto T, Basilico C
Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells
Somat Cell Mol Genet
1978
4:323-340
PubMed ID: 694723
DOI: 10.1007/BF01542846
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