Cell No. : Cell Name
RCB1267 : tsBN7
update : 2024/11/14
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| Comment | Temperature-sensitive mutant of BHK-21 with mutated DAD1 gene. Causing apoptosis at 39.5 C. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishimoto, Takeharu
|
| Originator |
Nishimoto, Takeharu
|
| Year of deposit |
1996
|
| Original cell |
BHK-21(C-13)
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Genus |
Mesocricetus
|
| Species |
auratus
|
| Gender |
Unknown
|
| Tissue |
kidney
|
| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_U473
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 10% CS
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
|
| SC frequency |
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Subculture : 1-2 times/2 weeks, Medium Renewal : 2 times/week
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| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, G6PD, PepB
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
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| Reference |
|
2134
Nakashima T, Sekiguchi T, Kuraoka A, Fukushima K, Shibata Y, Komiyama S, Nishimoto T
Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.
Mol Cell Biol
1993
13:6367-74
PubMed ID: 8413235
DOI: 10.1128/mcb.13.10.6367
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2132
Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M
Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells
Somat Cell Mol Genet
1982
8:811-824
PubMed ID: 6891837
DOI: 10.1007/BF01543021
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2133
Nishimoto T, Basilico C
Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells
Somat Cell Mol Genet
1978
4:323-340
PubMed ID: 694723
DOI: 10.1007/BF01542846
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