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Cell No. : Cell Name
RCB1264 : tsBN2  update : 2022/03/31
CommentTemperature-sensitive mutant of BHK-21. Causing premature chromosome condensation.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishimoto, Takeharu
Originator Nishimoto, Takeharu
Year of deposit 1996
Original cell BHK-21(C-13)
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Unknown
Tissue kidney
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U467
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, PepB
deposit info
lot info
Reference information Reference 7
User's Publication 1

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2138  Nishitani H, Ohtsubo M, Yamashita K, Iida H, Pines J, Yasudo H, Shibata Y, Hunter T, Nishimoto T  Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.  EMBO J  1991  10:1555-64  PubMed ID: 1851087   DOI: 10.1002/j.1460-2075.1991.tb07675.x
2139  Uchida S, Sekiguchi T, Nishitani H, Miyauchi K, Ohtsubo M, Nishimoto T  Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene.  Mol Cell Biol  1990  10:577-84  PubMed ID: 2300055   DOI: 10.1128/mcb.10.2.577
2140  Ohtsubo M, Kai R, Furuno N, Sekiguchi T, Sekiguchi M, Hayashida H, Kuma K, Miyata T, Fukushige S, Murotsu T, et al.  Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.  Genes Dev  1987  1:585-93  PubMed ID: 3678831   DOI: 10.1101/gad.1.6.585
2141  Kai R, Ohtsubo M, Sekiguchi M, Nishimoto T  Molecular cloning of a human gene that regulates chromosome condensation and is essential for cell proliferation.  Mol Cell Biol  1986  6:2027-32  PubMed ID: 3785187   DOI: 10.1128/mcb.6.6.2027
2132  Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M  Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells  Somat Cell Mol Genet  1982  8:811-824  PubMed ID: 6891837   DOI: 10.1007/BF01543021
2133  Nishimoto T, Basilico C  Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells  Somat Cell Mol Genet  1978  4:323-340  PubMed ID: 694723   DOI: 10.1007/BF01542846
2142  Nishimoto T, Eilen E, Basilico C  Premature of chromosome condensation in a ts DNA- mutant of BHK cells.  Cell  1978  15:475-83  PubMed ID: 719750   DOI: 10.1016/0092-8674(78)90017-x

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User's Publication
8554  Kimura T, Hashimoto I, Nishikawa M, Yamada H.  Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase.  Med Mol Morphol  2009  42:70-81  PubMed ID: 19536614   DOI: 10.1007/s00795-009-0441-3

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