Cell No. : Cell Name
RCB1264 : tsBN2
update : 2022/03/31
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| Comment | Temperature-sensitive mutant of BHK-21. Causing premature chromosome condensation. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishimoto, Takeharu
|
| Originator |
Nishimoto, Takeharu
|
| Year of deposit |
1996
|
| Original cell |
BHK-21(C-13)
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Genus |
Mesocricetus
|
| Species |
auratus
|
| Gender |
Unknown
|
| Tissue |
kidney
|
| Classification |
mutant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_U467
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, G6PD, PepB
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
7
|
| User's Publication |
1
|
| Reference |
|
2138
Nishitani H, Ohtsubo M, Yamashita K, Iida H, Pines J, Yasudo H, Shibata Y, Hunter T, Nishimoto T
Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.
EMBO J
1991
10:1555-64
PubMed ID: 1851087
DOI: 10.1002/j.1460-2075.1991.tb07675.x
|
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2139
Uchida S, Sekiguchi T, Nishitani H, Miyauchi K, Ohtsubo M, Nishimoto T
Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene.
Mol Cell Biol
1990
10:577-84
PubMed ID: 2300055
DOI: 10.1128/mcb.10.2.577
|
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2140
Ohtsubo M, Kai R, Furuno N, Sekiguchi T, Sekiguchi M, Hayashida H, Kuma K, Miyata T, Fukushige S, Murotsu T, et al.
Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.
Genes Dev
1987
1:585-93
PubMed ID: 3678831
DOI: 10.1101/gad.1.6.585
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2141
Kai R, Ohtsubo M, Sekiguchi M, Nishimoto T
Molecular cloning of a human gene that regulates chromosome condensation and is essential for cell proliferation.
Mol Cell Biol
1986
6:2027-32
PubMed ID: 3785187
DOI: 10.1128/mcb.6.6.2027
|
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2132
Nishimoto T, Sekiguchi T, Kai R, Yamashita K, Takahashi T, Sekiguchi M
Large-Scale Selection and Analysis of Temperature-Sensitive Mutants for Cell Reproduction from BHK Cells
Somat Cell Mol Genet
1982
8:811-824
PubMed ID: 6891837
DOI: 10.1007/BF01543021
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2133
Nishimoto T, Basilico C
Analysis of a Method for selecting Temperature-Sensitive Mutants of BHK Cells
Somat Cell Mol Genet
1978
4:323-340
PubMed ID: 694723
DOI: 10.1007/BF01542846
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2142
Nishimoto T, Eilen E, Basilico C
Premature of chromosome condensation in a ts DNA- mutant of BHK cells.
Cell
1978
15:475-83
PubMed ID: 719750
DOI: 10.1016/0092-8674(78)90017-x
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